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Literature summary for 2.8.2.32 extracted from
- Investigation of 5beta-scymnol sulfotransferases from the kidney and testis of Heterodontus portusjacksoni (1998), Comp. Biochem. Physiol. B, 121B, 243-249.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| inactivated by repeated thawing and freezing | Heterodontus portusjacksoni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 3',5'-diphosphate | 0.1 mM, 97% inhibition | Heterodontus portusjacksoni |  |
| iodoacetic acid | 0.1 mM, 93% inhibition | Heterodontus portusjacksoni | |
| NaN3 | 0.1 mM, 21% inhibition | Heterodontus portusjacksoni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | 25°C | Heterodontus portusjacksoni | |
| 0.013 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | 25°C | Heterodontus portusjacksoni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Heterodontus portusjacksoni | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 0.5 mM, 20% activation | Heterodontus portusjacksoni | |
| Mg2+ | 0.5 mM, 63% activation | Heterodontus portusjacksoni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Heterodontus portusjacksoni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol | Heterodontus portusjacksoni | - |
adenosine 3',5'-diphosphate + 5beta-scymnol sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heterodontus portusjacksoni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxylapatite, Sephadex G-100, partially purified | Heterodontus portusjacksoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Heterodontus portusjacksoni | - |
| testis | - |
Heterodontus portusjacksoni | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000000439 | - |
- |
Heterodontus portusjacksoni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, no loss of activity | Heterodontus portusjacksoni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name bbeta-scymnol sulfate | ? | |
| 3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | 150% of activity with 5beta-scymnol | Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | 62% of activity with 5beta-scymnol | Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol | - |
Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + 5beta-scymnol sulfate | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 37 | - |
Heterodontus portusjacksoni |
| 20 | 45 | - |
Heterodontus portusjacksoni |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Heterodontus portusjacksoni |
| 5.5 | 7 | - |
Heterodontus portusjacksoni |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00054 | - |
adenosine 3'-5'-diphosphate | - |
Heterodontus portusjacksoni | |
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