BRENDA - Enzyme Database show
show all sequences of 2.8.2.30

Role for 3-O-sulfated heparan sulfate as the receptor for herpes simplex virus type 1 entry into primary human corneal fibroblasts

Tiwari, V.; Clement, C.; Xu, D.; Valyi-Nagy, T.; Yue, B.Y.; Liu, J.; Shukla, D.; J. Virol. 80, 8970-8980 (2006)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
Homo sapiens
modification of the heparan sulfate of glycoprotein D gD receptors required for entry of Herpes simplex virus simplex 1, 3-OST-3 and the herpesvirus entry mediator are involved, but not nectin-1 or nectin-2, in primary croneal fibroblasts, a natural target cell type, while HeLa cells use nectin-1 as the entry receptor, overview, 3-OS HS is required for HSV-1 glycoprotein-induced cell-to-cell fusion of cultured corneal fibroblasts
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
?
additional information
Homo sapiens
HSV-1 glycoprotein-induced cell-to-cell fusion is inhibited by either prior treatment of cells with heparinases or by heparan sulfate preparations enriched in 3-OS HS, expression of HSV-1 gD in cultured corneal fibroblasts renders resistance to HSV-1 entry, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
corneal fibroblast cell line
3-OST-3 expression analysis
Homo sapiens
-
fibroblast
primary corneal, 3-OST-3 expression analysis
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
-
675705
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
3-OST-3 forms IdoUA2S-AnMan3S and IdoUA2S-AnMan3S6S disaccharides-containing products, product analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
modification of the heparan sulfate of glycoprotein D gD receptors required for entry of Herpes simplex virus simplex 1, 3-OST-3 and the herpesvirus entry mediator are involved, but not nectin-1 or nectin-2, in primary croneal fibroblasts, a natural target cell type, while HeLa cells use nectin-1 as the entry receptor, overview, 3-OS HS is required for HSV-1 glycoprotein-induced cell-to-cell fusion of cultured corneal fibroblasts
675705
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
additional information
HSV-1 glycoprotein-induced cell-to-cell fusion is inhibited by either prior treatment of cells with heparinases or by heparan sulfate preparations enriched in 3-OS HS, expression of HSV-1 gD in cultured corneal fibroblasts renders resistance to HSV-1 entry, overview
675705
Homo sapiens
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
Homo sapiens
modification of the heparan sulfate of glycoprotein D gD receptors required for entry of Herpes simplex virus simplex 1, 3-OST-3 and the herpesvirus entry mediator are involved, but not nectin-1 or nectin-2, in primary croneal fibroblasts, a natural target cell type, while HeLa cells use nectin-1 as the entry receptor, overview, 3-OS HS is required for HSV-1 glycoprotein-induced cell-to-cell fusion of cultured corneal fibroblasts
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
?
additional information
Homo sapiens
HSV-1 glycoprotein-induced cell-to-cell fusion is inhibited by either prior treatment of cells with heparinases or by heparan sulfate preparations enriched in 3-OS HS, expression of HSV-1 gD in cultured corneal fibroblasts renders resistance to HSV-1 entry, overview
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
corneal fibroblast cell line
3-OST-3 expression analysis
Homo sapiens
-
fibroblast
primary corneal, 3-OST-3 expression analysis
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
-
675705
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
3-OST-3 forms IdoUA2S-AnMan3S and IdoUA2S-AnMan3S6S disaccharides-containing products, product analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
modification of the heparan sulfate of glycoprotein D gD receptors required for entry of Herpes simplex virus simplex 1, 3-OST-3 and the herpesvirus entry mediator are involved, but not nectin-1 or nectin-2, in primary croneal fibroblasts, a natural target cell type, while HeLa cells use nectin-1 as the entry receptor, overview, 3-OS HS is required for HSV-1 glycoprotein-induced cell-to-cell fusion of cultured corneal fibroblasts
675705
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
additional information
HSV-1 glycoprotein-induced cell-to-cell fusion is inhibited by either prior treatment of cells with heparinases or by heparan sulfate preparations enriched in 3-OS HS, expression of HSV-1 gD in cultured corneal fibroblasts renders resistance to HSV-1 entry, overview
675705
Homo sapiens
?
-
-
-
-
Other publictions for EC 2.8.2.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738746
Sikora
Regulation of the expression o ...
Homo sapiens
J. Cell. Biochem.
117
1529-1542
2016
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739207
Mao
The heparan sulfate sulfotrans ...
Homo sapiens
Oncogene
35
5043-5055
2016
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1
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1
1
1
1
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737733
Zhao
Heparan sulfate 6-O-sulfotrans ...
Rattus norvegicus
Biochemistry
80
379-389
2015
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738745
Zhang
Heparan sulfate D-glucosaminyl ...
Homo sapiens
J. Cell. Biochem.
116
1101-1112
2015
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739264
Amraoui
Placental expression of hepara ...
Homo sapiens
Placenta
36
1218-1224
2015
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738938
Yakoub
Comprehensive analysis of herp ...
Danio rerio
J. Virol.
88
12915-12922
2014
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738961
Thacker
Heparan sulfate 3-O-sulfation: ...
Homo sapiens
Matrix Biol.
35
60-72
2014
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726083
Choudhary
Role of filopodia in HSV-1 Ent ...
Danio rerio
Open Virol. J.
7
41-48
2013
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1
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724808
Liu
Understanding the substrate sp ...
Homo sapiens
Curr. Opin. Struct. Biol.
22
550-557
2012
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725947
Nguyen
A synthetic heparan sulfate ol ...
Homo sapiens
Mol. Biosyst.
8
609-614
2012
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1
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726389
Moon
Dissecting the substrate recog ...
Mus musculus
Proc. Natl. Acad. Sci. USA
109
5265-5270
2012
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5
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724692
Song
Heparan sulfate D-glucosaminyl ...
Homo sapiens
Cancer Biol. Ther.
12
388-398
2011
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1
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703620
Bui
Epigenetics: methylation-assoc ...
Homo sapiens
FASEB J.
24
436-450
2010
1
1
1
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726527
Zhang
Heparin sulphate D-glucosaminy ...
Homo sapiens
Virology
406
280-285
2010
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6
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726544
Hubbard
Zebrafish-encoded 3-O-sulfotra ...
Danio rerio
Zebrafish
7
181-187
2010
-
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1
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690965
Copeland
Using a 3-O-sulfated heparin o ...
Human alphaherpesvirus 1, Human alphaherpesvirus 1 HSV-1
Biochemistry
47
5774-5783
2008
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674849
Vanpouille
The heparin/heparan sulfate se ...
Homo sapiens
J. Biol. Chem.
282
24416-24429
2007
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1
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691054
Wille
Biophysical investigation of h ...
Homo sapiens
Biochim. Biophys. Acta
1774
1470-1476
2007
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673246
Cadwallader
Combinatorial expression patte ...
Danio rerio
Dev. Dyn.
235
3423-3431
2006
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675705
Tiwari
Role for 3-O-sulfated heparan ...
Homo sapiens
J. Virol.
80
8970-8980
2006
-
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677244
O'Donnell
A role for heparan sulfate 3-O ...
Homo sapiens
Virology
346
452-459
2006
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1
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1
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2
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394962
Shworak
Multiple isoforms of heparan s ...
Homo sapiens
J. Biol. Chem.
274
5170-5184
1999
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1
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4
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1
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1
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1
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394963
Liu
Expression of heparan sulfate ...
Homo sapiens, Mus musculus
J. Biol. Chem.
274
5185-5192
1999
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1
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2
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2
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2
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1
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2
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2
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394964
Liu
Heparan sulfate D-glucosaminyl ...
Homo sapiens
J. Biol. Chem.
274
38155-38162
1999
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1
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1
1
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1
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1
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3
1
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1
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1
1
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1
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3
1
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394965
Shukla
A novel role for the 3-O-sulfa ...
Homo sapiens, Mus musculus
Cell
99
13-22
1999
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2
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2
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2
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2
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2
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