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Literature summary for 2.8.2.24 extracted from

  • Piotrowski, M.; Schemenewitz, A.; Lopukhina, A.; Muller, A.; Janowitz, T.; Weiler, E.W.; Oecking, C.
    Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure (2004), J. Biol. Chem., 279, 50717-50725.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli M15 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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ecotype C24, ecotype Col-0
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Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid-agarose Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
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isoenzyme AtST5a: best substrate: indole-3-methyl-desulfoglucosinolate, other substrates: benzyl-desulfoglucosinolate, 4-methylthiobutyl-desulfoglucosinolate, 4-methoxyindole-3-methyl-desulfoglucosinolate, N-methoxyindole-3-methyl-desulfoglucosinolate. pH 7.0, 30┬░C Arabidopsis thaliana
additional information
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isoenzyme AtST5b: best substrate: 7-methylthioheptyl-desulfoglucosinolate, other substrates: 8-methylthiooctyl-desulfoglucosinolate, 4-methylthiobutyl-desulfoglucosinolate, benzyl-desulfoglucosinolate, indole-3-methyl-desulfoglucosinolate, 8-methylsulfinyloctyl-desulfoglucosinolate, 3-butenyl-desulfoglucosinolate. pH 7.0, 30┬░C Arabidopsis thaliana
additional information
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isoenzyme AtST5c: best substrate: 8-methylthiooctyl-desulfoglucosinolate, other substrates: 7-methylthioheptyl-desulfoglucosinolate, benzyl-desulfoglucosinolate, 3-butenyl-desulfoglucosinolate, 4-methylthiobutyl-desulfoglucosinolate, 8-methylsulfinyloctyl-desulfoglucosinolate, 4-methylsulfinylbutyl-desulfoglucosinolate. pH 7.0, 30┬░C Arabidopsis thaliana