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Literature summary for 2.8.2.24 extracted from

  • Klein, M.; Reichelt, M.; Gershenzon, J.; Papenbrock, J.
    The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed (2006), FEBS J., 273, 122-136.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
G301D isoenzyme AtSOT18 C24, 25times higher specific activity with desulfobenzylglucosinolate Arabidopsis thaliana
N339K to date no purification of the protein under native conditions Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
3'-phosphoadenosine-5'-phosphate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information not detectable: 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT18 C24, pH 9.0, 37┬░C Arabidopsis thaliana
0.025
-
desulfobenzylglucosinolate isoenzyme AtSOT17 C24, pH 9.0, 37┬░C Arabidopsis thaliana
0.035
-
desulfobenzylglucosinolate isoenzyme AtSOT18 C24, pH 9.0, 37┬░C Arabidopsis thaliana
0.05
-
3'-phosphoadenosine-5'-phosphosulfate isoenzyme AtSOT18 Co10, pH 9.0, 37┬░C Arabidopsis thaliana
0.06
-
desulfobenzylglucosinolate isoenzyme AtSOT18 Co10, pH 9.0, 37┬░C Arabidopsis thaliana
0.1
-
3'-phosphoadenosine-5'-phosphosulfate isoenzyme AtSOT16 C24, pH 9.0, 37┬░C Arabidopsis thaliana
0.1
-
desulfobenzylglucosinolate isoenzyme AtSOT16 C24, pH 9.0, 37┬░C Arabidopsis thaliana
0.1
-
3'-phosphoadenosine-5'-phosphosulfate isoenzyme AtSOT17 C24, pH 9.0, 37┬░C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Arabidopsis thaliana 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39200
-
ecotype Co10, isoenzyme AtSOT16, calculated Arabidopsis thaliana
39900
-
ecotype Co10, isoenzyme AtSOT17, calculated Arabidopsis thaliana
40500
-
ecotype Co10, isoenzyme AtSOT18, calculated Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype C24, ecotype Co10
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
0.0016197 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT18 C24, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.007499 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT17 C24, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.008518 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT17 C24, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.01026 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT18 C24, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.0415 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT16 C24, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.05105 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT18 Co10, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.05159 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT18 Co10, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
0.06599 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT16 C24, pH 9.0, 37┬░C Arabidopsis thaliana
additional information
-
isoenzyme AtSOT16 C24 best substrate: 2-phenylethyl-glucosinolate Arabidopsis thaliana
additional information
-
isoenzyme AtSOT17 C24 best substrate: benzylglucosinolate Arabidopsis thaliana
additional information
-
isoenzyme AtSOT18 C24 best substrate: 6-methylthiohexylglucosinolate Arabidopsis thaliana
additional information
-
isoenzyme AtSOT18 Co10 best substrate: benzylglucosinolate Arabidopsis thaliana
additional information
-
isoenzyme AtSOT18G301D best substrate: 4-methylthiobutylglucosinolate Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine-5'-phosphosulfate + desulfobenzylglucosinolate
-
Arabidopsis thaliana adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Arabidopsis thaliana