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Literature summary for 2.8.2.24 extracted from

  • Glendening, T.M.; Poulton, J.E.
    Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum) (1990), Plant Physiol., 94, 811-816.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
bovine serum albumin, required for stabilizing Lepidium sativum
dilution inactivates Lepidium sativum
MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize Lepidium sativum

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 26% inhibition at 10 mM Lepidium sativum
adenosine-3',5'-bisphosphate product inhibition Lepidium sativum
Ag+ 13% inhibition at 1 mM Lepidium sativum
Cu2+ 14% inhibition at 1 mM Lepidium sativum
DTNB 2-mercaptoethanol protects Lepidium sativum
EDTA 15% inhibition at 10 mM Lepidium sativum
iodoacetamide weak inhibition Lepidium sativum
iodoacetic acid weak inhibition Lepidium sativum
additional information no inhibition by FeCl3, FeCl2, CaCl2, CoCl2 Lepidium sativum
N-ethylmaleimide 2-mercaptoethanol protects Lepidium sativum
Ni2+ 30% inhibition at 1 mM Lepidium sativum
o-phenanthroline 34% inhibition at 10 mM Lepidium sativum
p-chloromercuriphenylsulfonic acid 2-mercaptoethanol protects Lepidium sativum
Pb(NO3)2 25% inhibition at 1 mM Lepidium sativum
Zn2+ 46% inhibition at 1 mM Lepidium sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic studies Lepidium sativum
0.0065
-
desulfoallylglucosinolate pH 9.0, 30┬░C Lepidium sativum
0.06
-
3'-phosphoadenylylsulfate pH 9.0, 30┬░C Lepidium sativum
0.082
-
desulfobenzylglucosinolate pH 9.0, 30┬░C Lepidium sativum
0.67
-
desulfo-p-hydroxy-benzylglucosinolate pH 9.0, 30┬░C Lepidium sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 51% activation at 10 mM Lepidium sativum
Mn2+ 23% activation at 1 mM, 10 mM inhibits by 53% Lepidium sativum
additional information not affected by FeCl3, FeCl2, CaCl2, CoCl2 Lepidium sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
gel filtration Lepidium sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate Lepidium sativum
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
additional information Lepidium sativum involved in the biosynthesis of glucosinolates ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Brassica rapa subsp. oleifera
-
-
-
Lepidium sativum
-
no.5089, curled cress
-
Sinapis alba
-
-
-
Tropaeolum majus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
40.1fold Lepidium sativum

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin mechanism Lepidium sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated Arabidopsis thaliana
-
seedling etiolated Lepidium sativum
-
seedling etiolated Brassica rapa subsp. oleifera
-
seedling etiolated Sinapis alba
-
seedling etiolated Tropaeolum majus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.935
-
purified enzyme Lepidium sativum

Storage Stability

Storage Stability Organism
-20┬░C, 0.25 M sucrose and bovine serum albumin, 6 months Lepidium sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate
-
Lepidium sativum adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfoallylglucosinolate
-
Lepidium sativum adenosine 3',5'-bisphosphate + allylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS Lepidium sativum adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Arabidopsis thaliana adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Lepidium sativum adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica rapa subsp. oleifera adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Sinapis alba adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Tropaeolum majus adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
Lepidium sativum adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
additional information no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime Lepidium sativum ?
-
?
additional information involved in the biosynthesis of glucosinolates Lepidium sativum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000-55000, SDS-PAGE Lepidium sativum

Synonyms

Synonyms Comment Organism
ST
-
Lepidium sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
maximal activity in Tris-HCl buffer Lepidium sativum

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 about 65% of maximal activity at pH 7.0 and pH 9.5 Lepidium sativum

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
activity loss due to instability at pH values near the isoelectric point 5.2 Lepidium sativum

pI Value

Organism Comment pI Value Maximum pI Value
Lepidium sativum
-
-
5.2