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Literature summary for 2.8.2.22 extracted from

  • Kaysser, L.; Eitel, K.; Tanino, T.; Siebenberg, S.; Matsuda, A.; Ichikawa, S.; Gust, B.
    A new arylsulfate sulfotransferase involved in liponucleoside antibiotic biosynthesis in Streptomycetes (2010), J. Biol. Chem., 285, 12684-12694.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.22

Activating Compound

Activating Compound Comment Organism Structure
EDTA the addition of 0.1 mM EDTA increases product formation by 2.5fold Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3)pLys cells Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
H180L the mutant shows less than 5% activity compared to the wild type enzyme Streptomyces sp.
H253L the mutant shows less than 5% activity compared to the wild type enzyme Streptomyces sp.
H308L the mutant shows less than 5% activity compared to the wild type enzyme Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
phenol
-
 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0259
-
 phenol using 4-nitrophenyl sulfate as cosubstrate, at pH 6.7, temperature not specified in the publication Streptomyces sp.
0.0297
-
 phenol using methylumbelliferone sulfate as cosubstrate, at pH 6.7, temperature not specified in the publication Streptomyces sp.
0.0345
-
 methylumbelliferone sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.
0.0481
-
 4-nitrophenyl sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the presence of 20 mM Mg2+ or 2 mM Ni2ü in the assay leads to the precipitation of the protein Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. D6N7U5
-
-
Streptomyces sp. MK730-62F2 D6N7U5
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography, and Superdex 200 gel filtration Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + (5S)-5'-O-(5-amino-5-deoxy-beta-D-ribofuranosyl)-5'-C-[(2S,5S,6S)-5-carboxy-6-(hexadecanoyloxy)-4-methyl-3-oxo-1,4-diazepan-2-yl]uridine highly efficient substrate Streptomyces sp. ?
-
 ?
4-nitrophenyl sulfate + (5S)-5'-O-(5-amino-5-deoxy-beta-D-ribofuranosyl)-5'-C-[(2S,5S,6S)-5-carboxy-6-(hexadecanoyloxy)-4-methyl-3-oxo-1,4-diazepan-2-yl]uridine highly efficient substrate Streptomyces sp. MK730-62F2 ?
-
 ?
4-nitrophenyl sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. 4-nitrophenol + phenyl sulfate
-
 ?
4-nitrophenyl sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. MK730-62F2 4-nitrophenol + phenyl sulfate
-
 ?
methylumbelliferone sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. methylumbelliferone + phenyl sulfate
-
 ?
methylumbelliferone sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. MK730-62F2 methylumbelliferone + phenyl sulfate
-
 ?
additional information no product formation observed with 3'-phosphoadenosine 5'-phosphosulfate as the donor substrate Streptomyces sp. ?
-
 ?
additional information no product formation observed with 3'-phosphoadenosine 5'-phosphosulfate as the donor substrate Streptomyces sp. MK730-62F2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
arylsulfate sulfotransferase
-
 Streptomyces sp.
Cpz4
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 4-nitrophenyl sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.
0.15
-
 methylumbelliferone sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 7.6 maximal activity is observed at pH 6.7 with half-maximal values at pH 5.0 and 7.6 Streptomyces sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
41.7
-
 phenol using 4-nitrophenol sulfate as substrate, at pH 6.7, temperature not specified in the publication Streptomyces sp.
46.1
-
 phenol using methyl umbelliferone sulfate as substrate at pH 6.7, temperature not specified in the publication Streptomyces sp.