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Literature summary for 2.8.2.22 extracted from

  • Van Der Horst, M.; Van Lieshout, J.; Bury, A.; Hartog, A.; Wever, R.
    Sulfation of various alcoholic groups by an arylsulfate sulfotransferase from Desulfitobacterium hafniense and synthesis of estradiol sulfate (2012), Adv. Synth. Catal., 354, 3501-3508.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)cells Desulfitobacterium hafniense

Inhibitors

Inhibitors Comment Organism Structure
acetone in acetone the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense
dimethylformamide in dimethylformamide the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense
dioxane in dioxane the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense
tetrahydrofuran in tetrhydrofuran the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Desulfitobacterium hafniense
71400
-
x * 71400, calculated from amino acid sequence Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-nitrophenyl sulfate + phenol Desulfitobacterium hafniense 100% activity 4-nitrophenol + phenyl sulfate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO the enzyme is stable in the presence of 10% (v/v) of DMSO and even in 25% of DMSO, the remaining activity is still 733 Desulfitobacterium hafniense

Organism

Organism UniProt Comment Textmining
Desulfitobacterium hafniense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Poros column chromatography and Q-FF column chromatography Desulfitobacterium hafniense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + 1-butanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + butyl sulfate
-
?
4-nitrophenyl sulfate + 1-naphthol 50% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 1-naphththyl sulfate
-
?
4-nitrophenyl sulfate + 1-octanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + octyl sulfate
-
?
4-nitrophenyl sulfate + 1-pentanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + pentyl sulfate
-
?
4-nitrophenyl sulfate + 1-phenylethanol 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 1-phenylethyl sulfate
-
?
4-nitrophenyl sulfate + 17beta-estradiol after 24 h about 80% of the 17beta-estradiol is converted Desulfitobacterium hafniense 4-nitrophenol + ?
-
?
4-nitrophenyl sulfate + 2-aminophenol 136% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 2-aminophenyl sulfate
-
?
4-nitrophenyl sulfate + 2-butanol 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 2-butyl sulfate
-
?
4-nitrophenyl sulfate + 2-naphthol 86% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 2-naphthyl sulfate
-
?
4-nitrophenyl sulfate + 2-octanol 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 2-octyl sulfate
-
?
4-nitrophenyl sulfate + 2-phenylethanol 50% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 2-phenylethyl sulfate
-
?
4-nitrophenyl sulfate + 3,5-dimethoxyphenol 33% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 3,5-dimethoxyphenyl sulfate
-
?
4-nitrophenyl sulfate + 3-chlorophenol 138% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 3-chlorophenyl sulfate
-
?
4-nitrophenyl sulfate + 4-aminophenol 102% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-aminophenyl sulfate
-
?
4-nitrophenyl sulfate + 4-chlorophenol 195% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-chlorophenyl sulfate
-
?
4-nitrophenyl sulfate + 4-cresol 200% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-cresol sulfate
-
?
4-nitrophenyl sulfate + 4-hydroxybenzoic acid 0.7% activity compared to phenol Desulfitobacterium hafniense ?
-
?
4-nitrophenyl sulfate + 4-hydroxyindole 88% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + indol-4-yl sulfate
-
?
4-nitrophenyl sulfate + 5-hydroxyindole 74% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + indol-5-yl sulfate
-
?
4-nitrophenyl sulfate + benzyl alcohol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + benzyl sulfate
-
?
4-nitrophenyl sulfate + cyclohexanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + cyclohexyl sulfate
-
?
4-nitrophenyl sulfate + D-glucose 2-10% conversion in 7 days Desulfitobacterium hafniense ?
-
?
4-nitrophenyl sulfate + dihydroxyacetone 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + ?
-
?
4-nitrophenyl sulfate + dopamine 540% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + ?
-
?
4-nitrophenyl sulfate + estrone after 24 h 90% of the estrone is sulfated Desulfitobacterium hafniense 4-nitrophenol + estrone 3-sulfate
-
?
4-nitrophenyl sulfate + furfuryl alcohol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + furfuryl sulfate
-
?
4-nitrophenyl sulfate + glycerol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + ?
-
?
4-nitrophenyl sulfate + Leu-enkephalin within 48 h 91% of Leu-enkephalin is sulfated which reaches 98% after 6 days of incubation Desulfitobacterium hafniense 4-nitrophenol + Leu-enkephalin sulfate
-
?
4-nitrophenyl sulfate + N-hydroxysuccinimide 2-10% conversion in 7 days Desulfitobacterium hafniense ?
-
?
4-nitrophenyl sulfate + octyl glucoside 2-10% conversion in 7 days Desulfitobacterium hafniense ?
-
?
4-nitrophenyl sulfate + phenol 100% activity Desulfitobacterium hafniense 4-nitrophenol + phenyl sulfate
-
?
4-nitrophenyl sulfate + resorcinol 70% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + resorcinyl sulfate
-
?
4-nitrophenyl sulfate + tyramine 60% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-(2-aminoethyl)phenyl sulfate
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Desulfitobacterium hafniense
? x * 71400, calculated from amino acid sequence Desulfitobacterium hafniense

Synonyms

Synonyms Comment Organism
arylsulfate sulfotransferase
-
Desulfitobacterium hafniense
AST
-
Desulfitobacterium hafniense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
the melting temperature is at 47°C Desulfitobacterium hafniense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
-
Desulfitobacterium hafniense

pH Range

pH Minimum pH Maximum Comment Organism
9 10
-
Desulfitobacterium hafniense