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Literature summary for 2.8.2.22 extracted from

  • Kim, D.H.; Konishi, L.; Kobashi, K.
    Purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine (1986), Biochim. Biophys. Acta, 872, 33-41.
    View publication on PubMed

General Stability

General Stability Organism
4-nitrophenyl sulfate, stabilizes against thermal inactivation and against inactivation on storage under cold conditions Eubacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzenesulfonic acid 50% inhibition at 0.0000038 mM Eubacterium sp.
Cu2+ 50% inhibition at 0.01 mM Eubacterium sp.
diethyl dicarbonate 50% inhibition at 0.28 mM Eubacterium sp.
DTNB 50% inhibition at 0.023 mM Eubacterium sp.
EDTA inactivation reversed by addition of metal ions Eubacterium sp.
Fe2+ 50% inhibition at 0.6 mM Eubacterium sp.
Hg2+ 50% inhibition at 0.001 mM Eubacterium sp.
N-tosyl-L-lysylchloromethane 50% inhibition at 0.04 mM Eubacterium sp.
Ni2+ 50% inhibition at 1 mM Eubacterium sp.
PCMB 50% inhibition at 0.000025 mM Eubacterium sp.
Zn2+ 50% inhibition at 0.2 mM Eubacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.104
-
4-nitrophenyl sulfate with tyramine as second substrate, pH 8, 37°C Eubacterium sp.
3.5
-
tyramine with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C Eubacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ increases activity by 10-20% Eubacterium sp.
Mn2+ increases activity by 10-20% Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
4 * 80000 SDS-PAGE Eubacterium sp.
315000
-
gel filtration Eubacterium sp.

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
A-44
-
Eubacterium sp. A-44
-
A-44
-

Purification (Commentary)

Purification (Comment) Organism
-
Eubacterium sp.

Reaction

Reaction Comment Organism Reaction ID
an aryl sulfate + a phenol = a phenol + an aryl sulfate mechanism Eubacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
89.4
-
-
Eubacterium sp.

Storage Stability

Storage Stability Organism
4°C or -20°C, more than 50% loss of activity after 1 month Eubacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + phenol
-
Eubacterium sp. phenyl sulfate + 4-nitrophenol
-
?
4-nitrophenyl sulfate + phenol
-
Eubacterium sp. A-44 phenyl sulfate + 4-nitrophenol
-
?
4-nitrophenyl sulfate + phenolphthalein
-
Eubacterium sp. phenolphthalein O-sulfate + 4-nitrophenol
-
?
4-nitrophenyl sulfate + phenolphthalein
-
Eubacterium sp. A-44 phenolphthalein O-sulfate + 4-nitrophenol
-
?
4-nitrophenyl sulfate + salicylamide
-
Eubacterium sp. salicylamide O-sulfate + 4-nitrophenol
-
?
4-nitrophenyl sulfate + salicylamide
-
Eubacterium sp. A-44 salicylamide O-sulfate + 4-nitrophenol
-
?
4-nitrophenyl sulfate + tyramine
-
Eubacterium sp. 4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol i.e. tyramine O-sulfate ?
4-nitrophenyl sulfate + tyramine
-
Eubacterium sp. A-44 4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol i.e. tyramine O-sulfate ?

Subunits

Subunits Comment Organism
tetramer 4 * 80000 SDS-PAGE Eubacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Eubacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, pH 5.5-7, 50% loss of activity Eubacterium sp.
55
-
10 min, pH 5.5-7, complete loss of activity Eubacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
tyramine, salicylamide Eubacterium sp.
8.5
-
phenol Eubacterium sp.
9
-
phenolphthalein Eubacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7 most stable pH for storage Eubacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Eubacterium sp.
-
-
3.9