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Literature summary for 2.8.2.20 extracted from
- Tyrosine sulfation: an increasingly recognised post-translational modification of secreted proteins (2009), N. Biotechnol., 25, 299-317.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strongly stimulates isozyme TPST-2, but not TPST-1 | Homo sapiens |  |
| Mn2+ | strong activation of both isozymes | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozymes TPST-1 and TPST-2, DNA and amino acid sequence determinationand analysis, sequence comparison | Homo sapiens |
| isozymes TPST-1 and TPST-2. TPST-1 DNA and amino acid sequence determinationand analysis, sequence comparison | Mus musculus |
| TPST DNA and amino acid sequence determinationand analysis, sequence comparison | Drosophila melanogaster |
| TPST-1, sequence comparison | Rattus norvegicus |
| TPST-1, sequence comparison | Bos taurus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| determination of crystal and three dimensional structures of the isozymes | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of homozygous and heterozygous TPST-1 and/or TPST-2 knockout mutant mice. Disruption of one allele at the TPST-1 locus has no significant effect, and TPST-1+/- mice appear normal, have unaltered fertility and growth curves. TPST-1 -/- mice show reduced postnatal body weight. Disruption of the TPST-2 gene also causes no significant effect in TPST-2+/- mice, but TPST-2-/- mice also show reduced postnatal body weight like TPST-1 -/-mice, but reached normal body weight at 10 weeks of age. The most significant difference between TPST-1 -/- and TPST-2 -/- is the reduced fertility of TPST-2 knockout mice | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits both isozymes | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Drosophila melanogaster | 139 | - |
| Golgi membrane | - |
Mus musculus | 139 | - |
| Golgi membrane | - |
Rattus norvegicus | 139 | - |
| Golgi membrane | - |
Bos taurus | 139 | - |
| Golgi membrane | type II transmembrane isozymes | Homo sapiens | 139 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + C3aR tyrosine | Homo sapiens | C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF | adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + C5aR tyrosine | Homo sapiens | C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD | adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + CC receptor tyrosine | Homo sapiens | - |
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + CX3CR1 tyrosine | Homo sapiens | a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS | adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + FSHR tyrosine | Homo sapiens | follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335 | adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + LHR/CGR tyrosine | Homo sapiens | luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y | adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine | Homo sapiens | sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation | adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | Drosophila melanogaster | - |
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | Mus musculus | - |
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | Homo sapiens | - |
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | Bos taurus | - |
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + S1P1R tyrosine | Homo sapiens | type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D | adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + TSHR tyrosine | Homo sapiens | thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y | adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate | - |
? | |
| additional information | Homo sapiens | sulfation patterns, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Drosophila melanogaster | - |
isozymes TPST-1 and TPST-2 | - |
| Homo sapiens | - |
isozymes TPST-1 and TPST-2 | - |
| Mus musculus | - |
isozymes TPST-1 and TPST-2 | - |
| Rattus norvegicus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | enzymatic mechanism and structure-function relationships, overview. The catalytic mechanism follows the sequential steps: binding of PAPS, binding of the target protein, sulfate transfer, release of the sulfated target and release of 3',5'-ADP | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | - |
Homo sapiens | - |
| adrenal medulla | - |
Bos taurus | - |
| bladder | - |
Homo sapiens | - |
| blood | TPST-2 shows higher expression as TPST-1 | Homo sapiens | - |
| bone marrow | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| intestine | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| leukocyte | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| lung | - |
Homo sapiens | - |
| mammary gland | - |
Homo sapiens | - |
| additional information | wide distributed tissue expression pattern of isozymes TPST-1 and TPST-2, in several tissues, TPST-2 shows higher expression as TPST-1, expression pattern, overview | Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| pituitary gland | - |
Homo sapiens | - |
| prostate gland | - |
Homo sapiens | - |
| salivary gland | - |
Bos taurus | - |
| spleen | - |
Homo sapiens | - |
| testis | TPST-1 is expressed at substantially higher levels than TPST-2 | Homo sapiens | - |
| thymus | - |
Homo sapiens | - |
| thyroid gland | TPST-2 shows higher expression as TPST-1 | Homo sapiens | - |
| trachea | TPST-2 shows higher expression as TPST-1 | Homo sapiens | - |
| vascular tissue | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + C3aR tyrosine | C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF | Homo sapiens | adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + C5aR tyrosine | C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD | Homo sapiens | adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + CC receptor tyrosine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide | substrate is a synthetic modified mouse CCR8 peptide ADYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14, with Tyr15 last | Mus musculus | adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide | - |
? | |
| 3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide | substrate is a synthetic modified mouse CCR8 peptide DYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr14 is sulfated first, followed by Tyr3 and Tyr15 | Mus musculus | adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide | - |
? | |
| 3'-phosphoadenylyl sulfate + CX3CR1 tyrosine | a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS | Homo sapiens | adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + CX3CR1 tyrosine | a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS. Determination of sulfation sites by mutational analyis, overview. CX3CR1 has one known chemokine ligand, fractalkine and two tyrosine residues in its N-terminal region, Tyr14 and Tyr22 | Homo sapiens | adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + CXCR4 peptide tyrosine | a cytokine receptor peptide fragment | Homo sapiens | adenosine 3',5'-bisphosphate + CXCR4 peptide tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + FSHR tyrosine | follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335 | Homo sapiens | adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + LHR/CGR tyrosine | luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y | Homo sapiens | adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine | sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation | Homo sapiens | adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine | tyrosine sulfation of the amino-terminal region, structural basis of sulfotyrosine recognition, overview | Homo sapiens | adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Drosophila melanogaster | adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Mus musculus | adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + protein tyrosine | - |
Bos taurus | adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + S1P1R tyrosine | type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D | Homo sapiens | adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + TSHR tyrosine | thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y | Homo sapiens | adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate | - |
? | |
| additional information | sulfation patterns, overview | Homo sapiens | ? | - |
? | |
| additional information | enzyme activity with CCR receptors, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | TSPT-1 and TPST-2 are predicted to have type II transmembrane topology with a short 8-residue N-terminal cytoplasmic domain, an about 17-residue transmembrane domain and a luminal catalytic domain | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPST | - |
Rattus norvegicus |
| TPST | - |
Bos taurus |
| TPST-1 | - |
Drosophila melanogaster |
| TPST-1 | - |
Mus musculus |
| TPST-1 | - |
Homo sapiens |
| TPST-1 | - |
Rattus norvegicus |
| TPST-2 | - |
Drosophila melanogaster |
| TPST-2 | - |
Mus musculus |
| TPST-2 | - |
Homo sapiens |
| tyrosylprotein sulfotransferase | - |
Drosophila melanogaster |
| tyrosylprotein sulfotransferase | - |
Mus musculus |
| tyrosylprotein sulfotransferase | - |
Homo sapiens |
| tyrosylprotein sulfotransferase | - |
Rattus norvegicus |
| tyrosylprotein sulfotransferase | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
isozyme TPST-2 | Homo sapiens |
| 6.5 | - |
isozyme TPST-1 | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens | Drosophila melanogaster |
| physiological function | post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens | Mus musculus |
| physiological function | post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens | Rattus norvegicus |
| physiological function | post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens | Bos taurus |
| physiological function | post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens, physiological functions of protein sulfations, overview | Homo sapiens |
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