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Literature summary for 2.8.2.2 extracted from

  • Lyon, E.S.; Jakoby, W.B.
    The identity of alcohol sulfotransferases with hydroxysteroid sulfotransferases (1980), Arch. Biochem. Biophys., 202, 474-481.
    View publication on PubMed
Search for more literature for 2.8.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 dehydroepiandrosterone
-
 Rattus norvegicus
0.035
-
 beta-estradiol
-
 Rattus norvegicus
0.07
-
 testosterone
-
 Rattus norvegicus
0.17
-
 aldosterone pH 7.5, 37°C Rattus norvegicus
0.29
-
 cortisol pH 7.5, 37°C Rattus norvegicus
0.35
-
 estriol pH 7.5, 37°C Rattus norvegicus
0.44
-
 cortisol pH 7.5, 37°C Rattus norvegicus
0.52
-
 11-deoxycorticosterone pH 7.5, 37°C Rattus norvegicus
1
-
 Hexanol pH 7.5, 37°C Rattus norvegicus
1.2
-
 3-methyl-1-butanol pH 7.5, 37°C Rattus norvegicus
1.7
-
 1-Pentanol pH 7.5, 37°C Rattus norvegicus
3
-
 1-butanol
-
 Rattus norvegicus
17
-
 2-propanol pH 7.5, 37°C Rattus norvegicus
24
-
 1-propanol pH 7.5, 37°C Rattus norvegicus
42
-
 ethanol
-
 Rattus norvegicus
47
-
 ethanol pH 7.5, 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 6 * 28000, SDS-PAGE Rattus norvegicus
180000
-
 gel filtration Rattus norvegicus
180000
-
 sucrose density gradient centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-
Rattus norvegicus
-
 Sprague-Dawley, female
-

Purification (Commentary)

Purification (Comment) Organism
sulfotransferase 1 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
 substrate estradiol Rattus norvegicus
0.15
-
 substrate n-butanol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 1-butanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
-
 r
3'-phosphoadenylylsulfate + 1-hexanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-hexyl sulfate
-
 r
3'-phosphoadenylylsulfate + 1-pentanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-pentyl sulfate
-
 r
3'-phosphoadenylylsulfate + 1-propanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-propyl sulfate
-
 r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
-
 r
3'-phosphoadenylylsulfate + 2-propanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-propyl sulfate
-
 r
3'-phosphoadenylylsulfate + 3-methyl-1-butanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate
-
 r
3'-phosphoadenylylsulfate + aldosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + aldosterone sulfate
-
 r
3'-phosphoadenylylsulfate + beta-estradiol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate
-
 r
3'-phosphoadenylylsulfate + corticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + corticosterone sulfate
-
 r
3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol sulfate
-
 r
3'-phosphoadenylylsulfate + cortisone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisone sulfate
-
 r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
3'-phosphoadenylylsulfate + estriol poor substrate, relative activity 8% with beta-estradiol as 100% Rattus norvegicus adenosine 3',5'-bisphosphate + estriol sulfate
-
 r
3'-phosphoadenylylsulfate + ethanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ethyl sulfate
-
 r
3'-phosphoadenylylsulfate + methanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + methyl sulfate
-
 r
3'-phosphoadenylylsulfate + testosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + testosterone 3-sulfate
-
 r
additional information specificity of the enzyme is not limited to hydroxysteroids, a wide range of alcohols serve as sulfate acceptors Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 28000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
hydroxysteroid sulfotransferase 1
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0833
-
 methanol pH 7.5, 37°C Rattus norvegicus
0.133
-
 aldosterone pH 7.5, 37°C Rattus norvegicus
0.15
-
 cortisone pH 7.5, 37°C cosubstrate ethanol Rattus norvegicus
0.167
-
 estriol pH 7.5, 37°C Rattus norvegicus
0.183
-
 1-propanol pH 7.5, 37°C Rattus norvegicus
0.183
-
 cortisol pH 7.5, 37°C Rattus norvegicus
0.183
-
 11-deoxycorticosterone pH 7.5, 37°C Rattus norvegicus
0.217
-
 2-propanol pH 7.5, 37°C Rattus norvegicus
0.283
-
 1-butanol pH 7.5, 37°C Rattus norvegicus
0.417
-
 1-Hexanol pH 7.5, 37°C Rattus norvegicus
0.417
-
 3-methyl-1-butanol pH 7.5, 37°C Rattus norvegicus
0.45
-
 1-Pentanol pH 7.5, 37°C Rattus norvegicus
0.467
-
 testosterone pH 7.5, 37°C Rattus norvegicus
0.583
-
 beta-estradiol pH 7.5, 37°C Rattus norvegicus
2
-
 3beta-hydroxyandrost-5-ene-17-one pH 7.5, 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 beta-estradiol and dehydroepiandrosterone as substrate Rattus norvegicus
7.5
-
 1-butanol as substrate Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus preparative isoelectric focusing in sucrose gradients with ampholites in the range pH 3.0-8.0
-
 5