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Literature summary for 2.8.2.18 extracted from

  • Singer, S.S.; Gebhart, J.; Hess, E.
    Enzymatic sulfation of steroids. V. Partial purification and some properties of sulfotransferase III, the major glucocorticoid sulfotransferase of liver cytosol from male rats (1978), Can. J. Biochem., 56, 1028-1035.
    View publication on PubMed
Search for more literature for 2.8.2.18

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by adenine nucleotides Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 5 mM, strong inhibition Rattus norvegicus
dehydroepiandrosterone cortisol as substrate; kinetics Rattus norvegicus
dexamethasone cortisol as substrate; kinetics Rattus norvegicus
diethylstilbestrol cortisol as substrate Rattus norvegicus
additional information no inhibition by adenine nucleotides Rattus norvegicus
progesterone cortisol as substrate Rattus norvegicus
Zn2+ 5 mM, strong inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00628
-
 3'-phosphoadenylylsulfate pH 6.8, 37.5°C Rattus norvegicus
0.00682
-
 cortisol pH 6.8, 37.5°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activation Rattus norvegicus
Ba2+ 5 mM Rattus norvegicus
Ba2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
Ca2+ activation Rattus norvegicus
Ca2+ 5 mM Rattus norvegicus
Ca2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
Co2+ activation Rattus norvegicus
Co2+ 5 mM Rattus norvegicus
Co2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
Cr2+ activation Rattus norvegicus
Cr2+ 5 mM Rattus norvegicus
Cr2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
Mg2+ activation Rattus norvegicus
Mg2+ 5 mM Rattus norvegicus
Mg2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
Mn2+ activation Rattus norvegicus
Mn2+ 5 mM Rattus norvegicus
Mn2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
Ni2+ activation Rattus norvegicus
Ni2+ 5 mM Rattus norvegicus
Ni2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61500
-
 sulfotransferase III, gel filtration Rattus norvegicus
65000
-
 sedimentation velocity experiments Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + cortisol Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male CDR Fisher rats
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus
sulfotransferase III Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
additional information tissue distribution Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00098
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
3'-phosphoadenylylsulfate + cortisol i.e. PAPS, glucocorticoids are preferred substrates Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37.5
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 cortisol as substrate Rattus norvegicus