Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Chondroitin sulfates | competitive inhibitors | Bos taurus | |
lysozyme | stimulates 2fold at 1.5 mg/ml | Bos taurus | |
poly-L-lysine | stimulates 2fold at 1.5 mg/ml | Bos taurus | |
protamine | stimulates 2fold at 1.5 mg/ml | Bos taurus | |
spermidine | stimulates 2fold at 1 mM | Bos taurus | |
spermine | stimulates 2fold at 1 mM | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
chondroitin | - |
Bos taurus | |
0.48 | - |
dodecasaccharide | derived from chondroitin | Bos taurus | |
0.9 | - |
decasaccharide | derived from chondroitin | Bos taurus | |
2.5 | - |
Octasaccharide | derived from chondroitin | Bos taurus | |
5 | - |
hexasaccharide | derived from chondroitin | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required, Mn2+, Mg2+, Ca2+ | Bos taurus | |
Mg2+ | divalent cation required, Mn2+, Mg2+, Ca2+ | Bos taurus | |
Mn2+ | divalent cation required, Mn2+, Mg2+, Ca2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + chondroitin | Bos taurus | is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction | adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
calf | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
artery | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + decasaccharide | - |
Bos taurus | ? | - |
r | |
3'-phosphoadenylyl sulfate + dodecasaccharide | - |
Bos taurus | ? | - |
r | |
3'-phosphoadenylyl sulfate + hexasaccharide | - |
Bos taurus | ? | - |
r | |
3'-phosphoadenylyl sulfate + octasaccharide | - |
Bos taurus | ? | - |
r | |
3'-phosphoadenylylsulfate + chondroitin | specificity towards desulfated chondroitin sulfate and oligosaccharides derived therefrom | Bos taurus | adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate | - |
? | |
3'-phosphoadenylylsulfate + chondroitin | is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction | Bos taurus | adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate | - |
? | |
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides | even-numbered oligosaccharides with a glucuronic acid at the nonreducing terminus are active as acceptors, their capacity decreasing with decreasing chain length: dodecasaccharide at 64%, decasaccharide at 55%, octasaccharide at 17%, hexasaccharide at 4% of the chondroitin sulfation | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3 | Bos taurus |
7.3 | - |
double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3 | Bos taurus |