Literature summary for 2.8.2.15 extracted from

Senggunprai, L.; Yoshinari, K.; Yamazoe, Y.
Inhibitory effects of kynurenic acid, a tryptophan metabolite, and its derivatives on cytosolic sulfotransferases (2009), Biochem. J., 422, 455-462.

Search for more literature for 2.8.2.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mus musculus
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2,6-Dichloro-4-nitrophenol	non-specific SULT inhibitor	Homo sapiens
5,7-dichlorokynurenic acid	-	Homo sapiens
5,7-dichlorokynurenic acid	-	Mus musculus
gavestinel	i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt	Homo sapiens
gavestinel	i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt	Mus musculus
Kynurenic acid	exhibits selective inhibitory effects on Sult1b1-mediated sulfation of various compounds with IC50 values in the low micromolar range	Homo sapiens
Kynurenic acid	-	Mus musculus
L689,560	i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline	Homo sapiens
L689,560	i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline	Mus musculus
L701,324	i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone	Homo sapiens
L701,324	i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone	Mus musculus
Mefenamic acid	-	Homo sapiens
Mefenamic acid	-	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	1-naphthol	isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens
0.0051	-	dehydroepiandrosterone	isoform SULT2A1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Mus musculus
0.0063	-	xanthurenic acid	isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens
0.015	-	xanthurenic acid	isoform Sult1d1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Mus musculus
0.0209	-	3,4-dihydroxyphenylacetic acid	isoform Sult1d1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Mus musculus
0.0399	-	4-nitrophenol	isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens
0.145	-	L-triiodothyronine	isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens
0.206	-	xanthurenic acid	isoform SULT1C2, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens
0.238	-	3-(10,11-dihydro-5H-dibenzo[b,f]azepin-5-yl)-N-methylpropan-1-amine	isoform Sult2a1, in 50 mM Tris/HCl buffer, pH 10.0, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Mus musculus	5829	-
cytosol	-	Homo sapiens	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylyl sulfate + 1-naphthol	Mus musculus	-	adenosine 3',5'-bisphosphate + 1-naphthyl sulfate	-	?
3'-phosphoadenylyl sulfate + 1-naphthol	Homo sapiens	-	adenosine 3',5'-bisphosphate + 1-naphthyl sulfate	-	?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid	Mus musculus	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid	Homo sapiens	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + 4-nitrophenol	Mus musculus	-	adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate	-	?
3'-phosphoadenylyl sulfate + 4-nitrophenol	Homo sapiens	-	adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate	-	?
3'-phosphoadenylyl sulfate + beta-estradiol	Mus musculus	-	adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate	-	?
3'-phosphoadenylyl sulfate + beta-estradiol	Homo sapiens	-	adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate	-	?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	Mus musculus	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	Homo sapiens	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + desipramine	Mus musculus	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + desipramine	Homo sapiens	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + L-triiodothyronine	Mus musculus	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + L-triiodothyronine	Homo sapiens	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + xanthurenic acid	Mus musculus	-	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + xanthurenic acid	Homo sapiens	-	adenosine 3',5'-bisphosphate + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Mus musculus
Ni-NTA agarose column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + 1-naphthol	-	Mus musculus	adenosine 3',5'-bisphosphate + 1-naphthyl sulfate	-	?
3'-phosphoadenylyl sulfate + 1-naphthol	-	Homo sapiens	adenosine 3',5'-bisphosphate + 1-naphthyl sulfate	-	?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid	-	Mus musculus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid	-	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + 4-nitrophenol	-	Mus musculus	adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate	-	?
3'-phosphoadenylyl sulfate + 4-nitrophenol	-	Homo sapiens	adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate	-	?
3'-phosphoadenylyl sulfate + beta-estradiol	-	Mus musculus	adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate	-	?
3'-phosphoadenylyl sulfate + beta-estradiol	-	Homo sapiens	adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate	-	?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	-	Mus musculus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone	-	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + desipramine	-	Mus musculus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + desipramine	-	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + L-triiodothyronine	-	Mus musculus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + L-triiodothyronine	-	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + xanthurenic acid	-	Mus musculus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl sulfate + xanthurenic acid	-	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?

Synonyms

Synonyms	Comment	Organism
cytosolic sulfotransferase	-	Mus musculus
cytosolic sulfotransferase	-	Homo sapiens
SULT1B1	isoform	Homo sapiens
SULT1C2	isoform	Homo sapiens
SULT1D1	isoform	Mus musculus
SULT2A1	isoform	Mus musculus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0012	-	isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	gavestinel
0.0017	-	isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	gavestinel
0.005	-	IC50 above 0.005 mM, isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	Mefenamic acid
0.005	-	IC50 above 0.005 mM, isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	Mefenamic acid
0.0337	-	isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	Kynurenic acid
0.0342	-	isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	2,6-Dichloro-4-nitrophenol
0.0473	-	isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	L689,560
0.0536	-	isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	L689,560
0.1642	-	isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	5,7-dichlorokynurenic acid
0.2536	-	isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	5,7-dichlorokynurenic acid
9	-	isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	Kynurenic acid
24	-	isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C	Homo sapiens	2,6-Dichloro-4-nitrophenol

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Release 2023.1 (January 2023)