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Literature summary for 2.8.2.15 extracted from

  • Rouleau, M.; Marsolais, F.; Richard, M.; Nicolle, L.; Voigt, B.; Adam, G.; Varin, L.
    Inactivation of brassinosteroid biological activity by a salicylate-inducible steroid sulfotransferase from Brassica napus (1999), J. Biol. Chem., 274, 20925-20930.
    View publication on PubMed
Search for more literature for 2.8.2.15

Cloned(Commentary)

Cloned (Comment) Organism
genomic library constructed in pTZ18R, expression of recombinant BNST3 in Escherichia coli Brassica napus

Inhibitors

Inhibitors Comment Organism Structure
24-epicathasterone substrate inhibition above 0.005 mM Brassica napus
24-epiteasterone substrate inhibition above 0.005 mM Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 24-epicathasterone pH 7.5, 25°C Brassica napus
0.0042
-
 24-epiteasterone pH 7.5, 25°C Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
 cv. Westar
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + (22S,23S)-28-homobrassinolide
-
 Brassica napus adenosine 3',5'-bisphosphate + (22S,23S)-28-homobrassinolide sulfate
-
 r
3'-phosphoadenylylsulfate + (22S,23S)-28-homocastasterone
-
 Brassica napus adenosine 3',5'-bisphosphate + (22S,23S)-28-homocastasterone sulfate
-
 r
3'-phosphoadenylylsulfate + 17beta-estradiol
-
 Brassica napus adenosine 3',5'-bisphosphate + ?
-
 r
3'-phosphoadenylylsulfate + 17beta-estradiol 3-methyl ether
-
 Brassica napus adenosine 3',5'-bisphosphate + ?
-
 r
3'-phosphoadenylylsulfate + 24-epibrassinolide sulfonation leads to the loss of biological activity Brassica napus adenosine 3',5'-bisphosphate + 24-epibrassinolide sulfate
-
 r
3'-phosphoadenylylsulfate + 24-epicastasterone
-
 Brassica napus adenosine 3',5'-bisphosphate + 24-epicastasterone sulfate
-
 r
3'-phosphoadenylylsulfate + 24-epicastasterone highest affinity Brassica napus adenosine 3',5'-bisphosphate + 24-epicastasterone sulfate
-
 r
3'-phosphoadenylylsulfate + 24-epicastasterone
-
 Brassica napus adenosine 3',5'-bisphosphate + 24-epicastasterone 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + 24-epiteasterone
-
 Brassica napus adenosine 3',5'-bisphosphate + 24-epiteasterone sulfate
-
 r
3'-phosphoadenylylsulfate + 6-deoxo-24-epicastasterone
-
 Brassica napus adenosine 3',5'-bisphosphate + 6-deoxo-24-epicastasterone sulfate
-
 r
additional information little or no activity with brassinolide, (22S,23R)-28-homobrassinolide, and 22-deoxy-24-epiteasterone Brassica napus ?
-
 ?