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Literature summary for 2.8.2.15 extracted from

  • Sugiyama, Y.; Stolz, A.; Sugimoto, M.; Kuhlenkamp, J.; Yamada, T.; Kaplowitz, N.
    Identification and partial purification of a unique phenolic steroid sulphotransferase in rat liver cytosol (1984), Biochem. J., 224, 947-953.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.15

Inhibitors

Inhibitors Comment Organism Structure
estradiol substrate inhibition above 0.002 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 estradiol pH 7.6, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 1 * 32500 + 29500, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus
-
 male rats
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00071
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 17alpha-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
3'-phosphoadenylylsulfate + 17alpha-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 r
3'-phosphoadenylylsulfate + 17beta-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
3'-phosphoadenylylsulfate + 17beta-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 r
additional information oestrone 3-sulfate is no substrate, no taurolithocholate sulfotransferase activity Rattus norvegicus ?
-
 ?
additional information oestrone 3-sulfate is no substrate, no taurolithocholate sulfotransferase activity Rattus norvegicus Sprague-Dawley ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 32500 + 29500, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 substrate estradiol Rattus norvegicus
5.5 6
-
 Rattus norvegicus
6
-
 substrate estrone Rattus norvegicus
7 8 biphasic pH optimum, activity for estradiol, but not estrone Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Rattus norvegicus