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Literature summary for 2.8.2.14 extracted from
- Partial purification of a human liver sulphotransferase active towards bile salts (1980), Biochim. Biophys. Acta, 617, 192-204.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing reduces activity considerably, addition of ethylene glycol preserves activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Homo sapiens |  |
| Fe2+ | - |
Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
glycolithocholate | pH 7.5, 37°C | Homo sapiens | |
| 0.0033 | - |
3'-phosphoadenylylsulfate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | markedly activated by | Homo sapiens | |
| Mg2+ | markedly activated by | Homo sapiens | |
| Mn2+ | markedly activated by | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00163 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, ethylene glycol enzyme solution can be stored for at least 3 months without serious decrease in activity | Homo sapiens |
| 4°C, labile | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenosine-5'-phosphosulfate + oestrone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + estrone sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + chenodeoxycholate | sulfation at the 7-OH position | Homo sapiens | adenosine 3',5'-bisphosphate + chenodeoxycholate 7-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + deoxycholate | sulfation at the 12-OH position | Homo sapiens | adenosine 3',5'-bisphosphate + deoxycholate 12-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + glycolithocholate | - |
Homo sapiens | adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl-sulfate + phenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + phenyl sulfate | - |
? | |
| additional information | no bile salt disulfate formation detected | Homo sapiens | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | - |
5.4 |
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