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Literature summary for 2.8.2.11 extracted from
- Cerebroside sulfotransferase forms homodimers in living cells (2007), Biochemistry, 46, 9260-9269.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | inhibition of CST could be an option for the treatment of metachromatic leukodystrophy | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient or stable expression as GFP-tagged enzyme in CHO-K1 cells, expression of CST fused to a HA-tag or EGFP in BHK cells | Mus musculus |
| transient or stable expression of pEGFP-CST in CHO-K1 cell | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | minor part of recombinantly expressed enzyme | Mus musculus | 9986 | - |
| cell surface | only a minor fraction of the enzyme | Mus musculus | 9986 | - |
| Golgi apparatus | major part of the enzyme, not to the trans-Golgi network | Mus musculus | 5794 | - |
| Golgi apparatus | but no appearance in the trans-Golgi network | Mus musculus | 5794 | - |
| Golgi membrane | major part of the enzyme, the enzyme contains a transmembrane domain | Mus musculus | 139 | - |
| additional information | determination of subcellular localization, overview | Mus musculus | - |
- |
| plasma membrane | minor part of recombinantly expressed enzyme | Mus musculus | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
2 * 54000, recombinant HA-tagged enzyme, SDS-PAGE, CST forms homodimers, and dimerization is mediated by the lumenal domain of the enzyme, overview | Mus musculus |
| 110000 | - |
recombinant HA-tagged enzyme, analytical ultracentrifugation | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + a galactosylceramide | Mus musculus | - |
adenosine 3',5'-bisphosphate + a galactosylceramidesulfate | - |
? |  |
| additional information | Mus musculus | CST catalyzes the 3'-sulfation of galactose residues in several glycolipids, its major product in the mammalian brain is sulfatide, which is an essential myelin component | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Mus musculus | Q9JHE4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | two N-glycosylation sites at N66 and N312 are present and used | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + a galactosylceramide | - |
Mus musculus | adenosine 3',5'-bisphosphate + a galactosylceramidesulfate | - |
? | |
| additional information | CST catalyzes the 3'-sulfation of galactose residues in several glycolipids, its major product in the mammalian brain is sulfatide, which is an essential myelin component | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54000, recombinant HA-tagged enzyme, SDS-PAGE, CST forms homodimers, and dimerization is mediated by the lumenal domain of the enzyme, overview | Mus musculus |
| homodimer | calculated, glycerol gradient centrifugation in vitro and fluorescence correlation spectroscopy in vivo | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cerebroside sulfotransferase | - |
Mus musculus |
| CST | - |
Mus musculus |
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Release 2023.1 (January 2023)
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