Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.2.11 extracted from

  • Honke, K.; Yamane, M.; Ishii, A.; Kobayashi, T.; Makita, A.
    Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells (1996), J. Biochem., 119, 421-427.
    View publication on PubMed
Search for more literature for 2.8.2.11

Activating Compound

Activating Compound Comment Organism Structure
NaCl activation at concentration up to 0.1 M Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl inhibition at concentration above 0.1 M Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 3'-phosphoadenylylsulfate pH 6.5, 37°C Homo sapiens
0.027
-
 galactosylceramide pH 6.5, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 1 * 54000 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell renal cell carcinoma cell line, SMKT-R3 cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 20% glycerol, stable for 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactosyl 1-alkyl-2-acyl-sn-glycerol + 3'-phosphoadenylylsulfate
-
 Homo sapiens galactosyl 1-alkyl-2-acyl-sn-glycerol sulfate + adenosine 3',5'-bisphosphate
-
 ?
galactosyl diacylglycerol + 3'-phosphoadenylylsulfate
-
 Homo sapiens galactosyl diacylglycerol sulfate + adenosine 3',5'-bisphosphate
-
 ?
galactosylceramide + 3'-phosphoadenylylsulfate best substrate Homo sapiens galactosylceramide sulfate + adenosine 3',5'-bisphosphate
-
 ?
lactosylceramide + 3'-phosphoadenylylsulfate second best substrate Homo sapiens lactosylceramide sulfate + adenosine 3',5'-bisphosphate
-
 ?
additional information no substrate are globotriaosylceramide, galactose and lactose Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 54000 SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Homo sapiens