Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | up to 4 mM stimulates | Mus musculus | |
Triton X-100 | 0.4% Triton X-100 is optimal for enzyme activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulates | Mus musculus | |
Mg2+ | MgCl2 stimulates | Mus musculus | |
Mn2+ | MnCl2 stimulates | Mus musculus | |
NaCl | stimulates | Mus musculus |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | 0.4% Triton X-100 is optimal for enzyme activity | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in vitro assay | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactocerebroside + 3'-phosphoadenylylsulfate | - |
Mus musculus | galactocerebroside 3-sulfate + adenosine 3',5'-bisphosphate | - |
? | |
galactosylceramide + 3'-phosphoadenylylsulfate | biotinylated and light-sensitive azido derivatives of lysogalactosylceramide are synthesized by the crude enzyme preparation, these derivatives remain effective substrates for the testicular enzyme | Mus musculus | galactosylceramide sulfate + adenosine 3',5'-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
an optimized assay | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Mus musculus |