Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.2.10 extracted from

  • Nakamura, M.; Matsuda, K.; Morikawa, T.; Ishizuka, K.; Inouye, S.
    Efficient conversion to Cypridina luciferin from Cypridina luciferyl sulfate, coupled with enzymatic sulfation of acetic acid (2020), Biochem. Biophys. Res. Commun., 529, 678-684 .
    View publication on PubMed
Search for more literature for 2.8.2.10

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Vargula hilgendorfii
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + Renilla luciferin Vargula hilgendorfii
-
 adenosine 3',5'-bisphosphate + luciferyl sulfate
-
 r

Organism

Organism UniProt Comment Textmining
Vargula hilgendorfii
-
 i.e. Vargula hilgendorfii
-

Purification (Commentary)

Purification (Comment) Organism
partially by gel filtration and ultrafiltration Vargula hilgendorfii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + Renilla luciferin
-
 Vargula hilgendorfii adenosine 3',5'-bisphosphate + luciferyl sulfate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 25 assay at Vargula hilgendorfii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Vargula hilgendorfii

General Information

General Information Comment Organism
physiological function in Cypridina (Vargula) hilgendorfii, Cypridina luciferin is converted from Cypridina luciferyl sulfate by a sulfotransferase with adenosine 3',5'-diphosphate (PAP), and is used for the luminescence reaction of Cypridina luciferase. The luminescence activity of crude extracts of Cypridina hilgendorfii is significantly stimulated by the addition of acetic acid. This stimulation may be explained by an efficient supply of PAP from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) catalyzed by the sulfotransferase. Acetic acid acts as a sulfate acceptor from PAPS, followed by forming acetyl sulfate and PAP. The structure of acetyl sulfate is identified using mass spectrometry and it spontaneously decomposed to acetic acid and free sulfate ion in aqueous solutions. This enzymatic conversion from Cypridina luciferyl sulfate to Cypridina luciferin coupled with acetic acid and PAPS by the luciferin sulfotransferase, enzymatic cycling of Cypridina luciferyl sulfate to Cypridina luciferin coupled with acetic acid and PAPS Vargula hilgendorfii