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Literature summary for 2.8.2.10 extracted from

  • Anderson, J.M.; Hori, K.; Cormier, M.J.
    A bioluminescence assay for PAP(3',5'-diphosphoadenosine) and PAPS (3'-phosphoadenylyl sulfate) (1978), Methods Enzymol., 57, 244-257.
No PubMed abstract available

General Stability

General Stability Organism
activity is labile, can be stabilized by continous presence of 0.05 ml/l 2-mercaptoethanol Renilla reniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate Renilla reniformis
-
Renilla luciferin + 3'-phosphoadenylylsulfate
-
r

Organism

Organism UniProt Comment Textmining
Renilla reniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Renilla reniformis

Storage Stability

Storage Stability Organism
-80°C, requirement of 2-mercaptoethanol in storage buffer, stable for at least several months Renilla reniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl luciferin + 3'-phosphoadenylylsulfate fully active analog of coelenterate-type luciferin, equally effective as substrate Renilla reniformis benzyl luciferyl sulfate + adenosine 3',5'-bisphosphate
-
r
benzyl luciferyl sulfate + adenosine 3',5'-bisphosphate
-
Renilla reniformis benzyl luciferin + 3'-phosphoadenylylsulfate
-
r
Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
-
Renilla reniformis Renilla luciferin + 3'-phosphoadenylylsulfate
-
r