Literature summary for 2.8.2.10 extracted from

  • Nakamura, M.; Matsuda, K.; Nakamura, M.; Yamashita, K.; Suzuki, T.; Inouye, S.
    Enzymatic conversion of Cypridina luciferyl sulfate to Cypridina luciferin with coenzyme A as a sulfate acceptor in Cypridina (Vargula) hilgendorfii (2019), Photochem. Photobiol., 95, 1376-1386 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CoA CoA may be the competitive inhibitor against PAP in Cypridina sulfotransferase(s) and can also act as a sulfate acceptor Vargula hilgendorfii

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
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Vargula hilgendorfii
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + Renilla luciferin Vargula hilgendorfii
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adenosine 3',5'-bisphosphate + luciferyl sulfate
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r

Organism

Organism UniProt Comment Textmining
Vargula hilgendorfii
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i.e. Vargula hilgendorfii, collected at Chita (Aichi, Japan) on August 4, 2011, and Naruto (Tokushima, Japan) on August 24, 2017
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Purification (Commentary)

Purification (Comment) Organism
partially by gel filtration and ultrafiltration Vargula hilgendorfii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CoA can act as a sulfate acceptor in the sulfotransferase reaction, and can also competitively inhibit the sulfotransferase. Sulfotransferase(s) in Cypridina specimens can efficiently use CoA, 5'-AMP and 3'-AMP as a sulfate acceptor from Cypridina luciferyl sulfate and produce Cypridina luciferin, similar to the case with PAP. Mass spectral analyses of the sulfated products of 5'-AMP, 3'-AMP, and CoA Vargula hilgendorfii ?
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3'-phosphoadenylyl sulfate + Renilla luciferin
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Vargula hilgendorfii adenosine 3',5'-bisphosphate + luciferyl sulfate
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r
3'-AMP + luciferyl sulfate
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Vargula hilgendorfii 3'-AMP sulfate + luciferin
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?
5'-AMP + luciferyl sulfate
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Vargula hilgendorfii 5'-AMP sulfate + luciferin
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?
CoA + luciferyl sulfate
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Vargula hilgendorfii CoA sulfate + luciferin
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 25 assay at Vargula hilgendorfii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 assay at Vargula hilgendorfii

General Information

General Information Comment Organism
physiological function in the luminous ostracod Cypridina (presently Vargula) hilgendorfii, Cypridina luciferyl sulfate (3-enol sulfate of Cypridina luciferin) is converted to Cypridina luciferin by a sulfotransferase with 3'-phosphoadenosine-5'-phosphate (PAP) as a sulfate acceptor. The resultant Cypridina luciferin is used for the luciferase-luciferin reaction of Cypridina to emit blue light. the addition of CoA to the crude extracts significantly stimulates luminescence intensity. CoA can act as a sulfate acceptor from Cypridina luciferyl sulfate. In addition, the sulfate group of Cypridina luciferyl sulfate is also transferred to adenosine 5'-monophosphate (5'-AMP) and adenosine 3'-monophosphate (3'-AMP) by the sulfotransferase. Other adenosine derivatives of 5'-AMP and 3'-AMP can also act as efficient sulfate acceptors on the millimolar order from Cypridina luciferyl sulfate by the sulfotransferase and they stimulate light emission in crude extracts of Cypridina specimens. ATP, NADH, and NAD+ weakly stimulate the luminescence activity Vargula hilgendorfii