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Literature summary for 2.8.2.1 extracted from

  • Berger, I.; Guttman, C.; Amar, D.; Zarivach, R.; Aharoni, A.
    The molecular basis for the broad substrate specificity of human sulfotransferase 1A1 (2011), PLoS ONE, 6, e26794.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with adenosine 3',5'-bisphosphate alone, with adenosine 3',5'-bisphosphate and 3-cyano-7-hydroxycumarin and with adenosine 3',5'-bisphosphate and 2-naphthol, vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
D249G the mutant shows higher specific activity towards 3-cyano-7-hydroxycumarin (0.1 mM and above) compared to the wild type enzyme. The mutant is less heat stable than the wild type enzyme Homo sapiens
L67V/A101S/Q177K/V211L/F222K/F247I the mutant is more heat stable than the wild type enzyme Homo sapiens
Q56E/A101S/T117S/H213R/F222L/V243L/T266N/L111P/Y240C the mutant is more heat stable than the wild type enzyme Homo sapiens
Q56E/A101S/T117S/Q177K/M223I/V243L the mutant shows higher specific activity towards 3-cyano-7-hydroxycumarin (0.1 mM and above) compared to the wild type enzyme. The mutant is more heat stable than the wild type enzyme Homo sapiens
Q56E/L67V/A101S/T117S/H213R/F222L/V243I/D249G the mutant shows higher specific activity towards 3-cyano-7-hydroxycumarin (0.1 mM and above) compared to the wild type enzyme. The mutant is more heat stable than the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 3-cyano-7-hydroxycoumarin wild type enzyme, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P50225
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + 2-naphthol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
3'-phosphoadenylyl sulfate + 3-cyano-7-hydroxycoumarin
-
 Homo sapiens ?
-
 ?
3'-phosphoadenylyl sulfate + 4-nitrophenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?

Synonyms

Synonyms Comment Organism
sulfotransferase 1A1
-
 Homo sapiens
SULT1A1
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the wild type enzyme remains stable up to 40°C and then rapidly loses activity being completely inactive at temperatures of 42°C and above Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.149
-
 3-cyano-7-hydroxycoumarin mutant enzyme Q56E/A101S/T117S/Q177K/M223I/V243L, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens
0.308
-
 3-cyano-7-hydroxycoumarin mutant enzyme D249G, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens
0.466
-
 3-cyano-7-hydroxycoumarin mutant enzyme Q56E/L67V/A101S/T117S/H213R/F222L/V243I/D249G, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens
1.2
-
 3-cyano-7-hydroxycoumarin wild type enzyme, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens