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Literature summary for 2.8.2.1 extracted from

  • Sakakibara, Y.; Yanagisawa, K.; Takami, Y.; Nakayama, T.; Suiko, M.; Liu, M.C.
    Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases (1998), Biochem. Biophys. Res. Commun., 247, 681-686.
    View publication on PubMed
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type P-PST and M-PST and chimeric mutants bearing parts of both enzyme forms in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of several chimeric mutants by exchange of sequence fragments between the P-PST and the M-PST form, the structural determinants for the substrate specificity of the 2 forms comprsie the internal domain-spanning amino acid residues 84-148 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, chimeric enzymes Homo sapiens
0.00036
-
 4-nitrophenol recombinant wild-type P-PST, pH 7.0, 37°C Homo sapiens
0.0014
-
 dopamine recombinant wild-type M-PST, pH 7.0, 37°C Homo sapiens
0.0875
-
 dopamine recombinant wild-type P-PST, pH 7.0, 37°C Homo sapiens
0.167
-
 4-nitrophenol recombinant wild-type M-PST, pH 7.0, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates M-PST about 3.5fold, with substrate D-tyrosine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43704 2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type P-PST and M-PST and chimeric mutants Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-nitrophenol M-PST Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-nitrophenol P-PST Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine i.e. D-Dopa Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + D-tyrosine M-PST Homo sapiens adenosine 3',5'-bisphosphate + D-tyrosyl sulfate
-
 ?
3'-phosphoadenylylsulfate + DL-tyrosine M-PST Homo sapiens adenosine 3',5'-bisphosphate + DL-tyrosyl sulfate
-
 ?
3'-phosphoadenylylsulfate + dopamine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + dopamine P-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine i.e. L-dopa Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine M-PST Homo sapiens adenosine 3',5'-bisphosphate + L-tyrosyl O4-sulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens