Cloned (Comment) | Organism |
---|---|
SULT1A1, DNA sequence determination and analysis, expression in Escherichia coli | Homo sapiens |
SULT1A3, wild-type and mutants, expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E146A | site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 | Homo sapiens |
H143Y | site-directed mutagenesis, reduced activity with dopamine compared to wild-type SULT1A3 | Homo sapiens |
H143Y/E146A | site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
3'-phosphoadenylylsulfate | SULT1A3 mutant H143Y, pH 7.4, 37°C | Homo sapiens | |
0.0022 | - |
dopamine | wild-type SULT1A3, pH 7.4, 37°C | Homo sapiens | |
0.003 | - |
3'-phosphoadenylylsulfate | SULT1A3 mutant E146A, pH 7.4, 37°C | Homo sapiens | |
0.0038 | - |
4-nitrophenol | pH 7.4, 37°C | Homo sapiens | |
0.0038 | - |
4-nitrophenol | wild-type SULT1A1 | Homo sapiens | |
0.0043 | - |
3'-phosphoadenylylsulfate | wild-type SULT1A1, pH 7.4, 37°C | Homo sapiens | |
0.0046 | - |
3'-phosphoadenylylsulfate | wild-type SULT1A3, pH 7.4, 37°C | Homo sapiens | |
0.0048 | - |
3'-phosphoadenylylsulfate | SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C | Homo sapiens | |
0.0056 | - |
dopamine | SULT1A3 mutant H143Y, pH 7.4, 37°C | Homo sapiens | |
0.0061 | - |
4-nitrophenol | SULT1A3 mutant E146A, pH 7.4, 37°C | Homo sapiens | |
0.0094 | - |
4-nitrophenol | SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C | Homo sapiens | |
0.087 | - |
dopamine | SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C | Homo sapiens | |
0.109 | - |
dopamine | wild-type SULT1A1, pH 7.4, 37°C | Homo sapiens | |
0.127 | - |
dopamine | SULT1A3 mutant E146A, pH 7.4, 37°C | Homo sapiens | |
1.38 | - |
4-nitrophenol | wild-type SULT1A3, pH 7.4, 37°C | Homo sapiens | |
1.47 | - |
4-nitrophenol | SULT1A3 mutant H143Y, pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
SULT1A3, is a M-PST enzyme form | - |
Homo sapiens | P50225 | SULT1A1 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Homo sapiens |
recombinant wild-type SULT1A3 and mutants from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate | Glu146 determines the substrate specificity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | SULT1A1 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + a phenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + dopamine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + dopamine | SULT1A3 is specific for dopamine and other biogenic amines | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
additional information | substrate specificity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SULT | - |
Homo sapiens |