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Literature summary for 2.8.2.1 extracted from

  • Hwang, S.R.; Palkovits, M.; Hook, V.Y.
    High level expression and characterization of recombinant human hippocampus phenol sulfotransferase: a novel phenol-sulfating form of phenol sulfotransferase (1997), Protein Expr. Purif., 11, 125-134.
    View publication on PubMed
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of H-PST in Escherichia coli BL21(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 4-nitrophenol H-PST, pH 6.8, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, recombinant H-PST, SDS-PAGE Homo sapiens
60000 65000 H-PST, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 hippocampal enzyme form H-PST
-

Purification (Commentary)

Purification (Comment) Organism
recombinant H-PST from Escherichia coli, 14.5fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain H-PST Homo sapiens
-
hippocampus H-PST Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.054
-
 purified recombinant H-PST Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-nitrophenol H-PST, preferred substrate Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + dopamine H-PST, no activity with Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + epinephrine H-PST, no activity Homo sapiens adenosine 3',5'-bisphosphate + epinephrine sulfate
-
 ?
additional information hippocampal H-PST is not active with neuropeptide substrates Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, recombinant H-PST, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
H-PST i.e. hippocampal phenol sulfotransferase Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 H-PST, inactivation after 15 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Homo sapiens