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Literature summary for 2.8.2.1 extracted from

  • Whittemore, R.M.; Pearce, L.B.; Roth, J.A.
    Purification and kinetic characterization of a dopamine-sulfating form of phenol sulfotransferase from human brain (1985), Biochemistry, 24, 2477-2482.
    View publication on PubMed
Search for more literature for 2.8.2.1

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-bisphosphate
-
 Homo sapiens
ATP M-PST Homo sapiens
NaCl 50% inhibition at: 325 mM; M-PST Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 3'-phosphoadenylylsulfate M-PST, pH 7.0, 37°C Homo sapiens
0.0029
-
 dopamine M-PST, pH 7.0, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 isozyme M-PST, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST
-

Purification (Commentary)

Purification (Comment) Organism
monoamine-sulfating form, M-PST Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate isozyme M-PST: ordered bisubstrate reaction mechanism Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
0.0077
-
 purified M-PST Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-methoxytyramine
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + dopamine
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + tyramine
-
 Homo sapiens adenosine 3',5'-bisphosphate + tyramine O-sulfate
-
 ?
additional information substrate specificity Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 M-PST, with substrate dopamine Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
 adenosine 3',5'-bisphosphate M-PST, pH 7.0, 37°C Homo sapiens
0.18
-
 ATP M-PST, pH 7.0, 37°C Homo sapiens