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Literature summary for 2.8.2.1 extracted from
- Rat brain phenolsulfotransferase: partial purification and some properties (1973), Biochim. Biophys. Acta, 327, 365-374.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
dihydroxyphenylacetic acid | pH 5.8, 37°C | Rattus norvegicus |  |
| 0.014 | - |
homovanillic acid | pH 5.8, 37°C | Rattus norvegicus | |
| 0.017 | - |
3,4-dihydroxyphenylglycol | pH 6.0, 37°C | Rattus norvegicus | |
| 0.018 | - |
3-methoxy-4-hydroxyphenylglycol | pH 6.4, 37°C | Rattus norvegicus | |
| 0.02 | - |
dopamine | pH 9.0, 37°C | Rattus norvegicus | |
| 0.04 | - |
phenol | pH 5.8, 37°C | Rattus norvegicus | |
| 0.1 | - |
vanillylmandelic acid | pH 6.2, 37°C | Rattus norvegicus | |
| 0.1 | - |
norepinephrine | pH 9.0, 37°C | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 30fold from brain | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 7 days, 20% loss of activity of partially purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + a phenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylacetic acid | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylglycol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + dopamine | low activity | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + homovanillic acid | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + norepinephrine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + norepinephrine sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + phenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + phenyl sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + vanillylmandelic acid | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Rattus norvegicus |
| 6 | - |
with substrate 3,4-dihydroxyphenylglycol | Rattus norvegicus |
| 6.2 | - |
with substrate vanillylmandelic acid | Rattus norvegicus |
| 6.4 | - |
with substrate 3-methoxy-4-hydroxyphenylglycol | Rattus norvegicus |
| 9 | - |
with substrate dopamine or norepinephrine | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
4°C, 82 h, 60% loss of activity | Rattus norvegicus |
| 8 | - |
4°C, 82 h, 80% loss of activity | Rattus norvegicus |
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