Literature summary for 2.8.1.9 extracted from

Lehrke, M.; Rump, S.; Heidenreich, T.; Wissing, J.; Mendel, R.R.; Bittner, F.
Identification of persulfide-binding and disulfide-forming cysteine residues in the NifS-like domain of the molybdenum cofactor sulfurase ABA3 by cysteine-scanning mutagenesis (2012), Biochem. J., 441, 823-832.

Search for more literature for 2.8.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DL41 cells	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
C101/C430A	inactive	Arabidopsis thaliana
C151/C430A	inactive	Arabidopsis thaliana
C206S	the mutants activity is reduced to 18%	Arabidopsis thaliana
C206S/C430A	inactive	Arabidopsis thaliana
C250/C430A	inactive	Arabidopsis thaliana
C377/C430A	inactive	Arabidopsis thaliana
C428/435A	the mutant shows wild type sensitivity towards N-ethylmaleimide	Arabidopsis thaliana
C435A	the mutant is less sensitive towards N-ethylmaleimide compared to the wild type enzyme	Arabidopsis thaliana
C456/C430A	inactive	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
N-ethylmaleimide	less than 80% activity at 1 mM, 30% activity at 1.5 mM, complete inhibition at 2 mM	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
molybdenum cofactor + L-cysteine + H+	Arabidopsis thaliana	the enzyme consists of an N-terminal NifS-like domain that exhibits L-cysteine desulfurase activity and a C-terminal domain that binds sulfurated molybdenum cofactor	thio-molybdenum cofactor + L-alanine + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9C5X8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
molybdenum cofactor + L-cysteine + H+	the enzyme consists of an N-terminal NifS-like domain that exhibits L-cysteine desulfurase activity and a C-terminal domain that binds sulfurated molybdenum cofactor	Arabidopsis thaliana	thio-molybdenum cofactor + L-alanine + H2O	-	?

Synonyms

Synonyms	Comment	Organism
ABA3	-	Arabidopsis thaliana
cysteine desulfurase	-	Arabidopsis thaliana
molybdenum cofactor sulfurase	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)