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Literature summary for 2.8.1.9 extracted from
- Characterization of the NifS-like domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration (2005), J. Biol. Chem., 280, 4213-4218.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in vector pQE80 | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C430A | ABA3-NifS, reduced activity to 14% for L-cysteine and 20% for L-selenocysteine | Arabidopsis thaliana |
| K271S | ABA3-NifS, inactive, unable to bind pyridoxal 5'-phosphate | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
L-cysteine | for the L-cysteine desulfurase domain, 2 mM DTT, pH 9.3, 37°C | Arabidopsis thaliana |  |
| 0.2 | - |
L-selenocysteine | for the L-cysteine desulfurase domain, 2 mM DTT, pH 9.3, 37°C | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| MoO2(OH)Dtpp-mP + L-cysteine + 2 H+ | Arabidopsis thaliana | - |
MoOS(OH)Dtpp-mP + L-alanine + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9C5X8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-nitrilotriacetic acid superflow matrix, UNO Q-1 column, Superose 12 size exclusion column | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine | - |
Arabidopsis thaliana | L-alanine + H2S | in presence of DTT as reducing agent, activity of the the cysteine desulfurase domain | ? | |
| L-cysteine methyl ester | - |
Arabidopsis thaliana | L-alanine methyl ester + H2S | in presence of DTT as reducing agent, activity of the the cysteine desulfurase domain | ? | |
| L-selenocysteine | - |
Arabidopsis thaliana | L-alanine + H2Se | in presence of DTT as reducing agent, activity of the the cysteine desulfurase domain | ? | |
| MoO2(OH)Dtpp-mP + L-cysteine + 2 H+ | - |
Arabidopsis thaliana | MoOS(OH)Dtpp-mP + L-alanine + H2O | - |
? | |
| additional information | no detectable activity for L-cysteine ethylester, glutathione, beta-mercaptopyruvate, L-cysteamine, and L-cystine | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABA3 | - |
Arabidopsis thaliana |
| molybdenum cofactor sulfurase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
L-cysteine | for the L-cysteine desulfurase domain, 2 mM DTT, pH 9.3, 37°C | Arabidopsis thaliana | |
| 0.357 | - |
L-selenocysteine | for the L-cysteine desulfurase domain, 2 mM DTT, pH 9.3, 37°C | Arabidopsis thaliana | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | molybdenum cofactor biosynthesis | Arabidopsis thaliana |
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