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Literature summary for 2.8.1.7 extracted from
- Kinetic analysis of the bisubstrate cysteine desulfurase SufS from Bacillus subtilis (2010), Biochemistry, 49, 8794-8802.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli CL100 cells | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C361A | the mutant is able to interact with SufU and to equally binds cysteine, but it does not generate alanine or sulfide in the presence of SufU, cysteine, and dithiothreitiol | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
[SufU]-cysteine | in 50 mM MOPS (pH 7.4), temperature not specified in the publication | Bacillus subtilis | |
| 0.086 | - |
L-cysteine | in 50 mM MOPS (pH 7.4), temperature not specified in the publication | Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, calculated from amino acid sequence | Bacillus subtilis |
| 88000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis PS832 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography and Sephacryl S-200 gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + [SufU]-cysteine | - |
Bacillus subtilis | L-alanine + [SufU]-S-sulfanylcysteine | - |
? | |
| L-cysteine + [SufU]-cysteine | - |
Bacillus subtilis PS832 | L-alanine + [SufU]-S-sulfanylcysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, calculated from amino acid sequence | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cysteine:SufU sulfurtransferase | - |
Bacillus subtilis |
| SufS | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.87 | - |
L-cysteine | in 50 mM MOPS (pH 7.4), temperature not specified in the publication | Bacillus subtilis | |
| 0.87 | - |
[SufU]-cysteine | in 50 mM MOPS (pH 7.4), temperature not specified in the publication | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Bacillus subtilis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.1 | - |
L-cysteine | in 50 mM MOPS (pH 7.4), temperature not specified in the publication | Bacillus subtilis | |
| 290 | - |
[SufU]-cysteine | in 50 mM MOPS (pH 7.4), temperature not specified in the publication | Bacillus subtilis |
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