Literature summary for 2.8.1.7 extracted from
WU, A.; ZHANG, Y.; ZHENG, C.; DAI, Y.; LIU, Y.; ZENG, J.; GU, G.; LIU, J.
Purification and enzymatic characteristics of cysteine desulfurase, IscS, in Acidithiobacillus ferrooxidans ATCC 23270 (2008), Trans. Nonferrous Met. Soc. China, 18, 1450-1457.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Escherichia coli BL21(DE3) cells |
Acidithiobacillus ferrooxidans |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine |
up to 90% inhibition at 5 mM |
Acidithiobacillus ferrooxidans |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.11 |
- |
L-cysteine |
in 50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 5 mM dithiothreitol, at 25°C |
Acidithiobacillus ferrooxidans |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
46000 |
- |
SDS-PAGE |
Acidithiobacillus ferrooxidans |
Organism
Organism |
UniProt |
Comment |
Textmining |
Acidithiobacillus ferrooxidans |
- |
strain ATCC 23270 |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
Hi-Trap column chromatography |
Acidithiobacillus ferrooxidans |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
0.0224 |
- |
crude extract, at 25°C |
Acidithiobacillus ferrooxidans |
0.148 |
- |
enzyme after purification, at 25°C |
Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
L-cysteine + [enzyme]-cysteine |
IscS has a high affinity for L-cysteine |
Acidithiobacillus ferrooxidans |
L-alanine + [enzyme]-S-sulfanylcysteine |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
IscS |
- |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
30 |
- |
- |
Acidithiobacillus ferrooxidans |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
40 |
55 |
the enzyme is relatively stable at 40°C with 67% loss of activity, and unstable at temperatures above 55°C |
Acidithiobacillus ferrooxidans |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
8 |
8.5 |
- |
Acidithiobacillus ferrooxidans |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
7 |
9 |
- |
Acidithiobacillus ferrooxidans |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
5 |
10 |
about 45% residual activity at pH 7.5 and 9.5, almost no activity at pH 5.0 and 10.0 |
Acidithiobacillus ferrooxidans |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
pyridoxal 5'-phosphate |
- |
Acidithiobacillus ferrooxidans |
|