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Literature summary for 2.8.1.7 extracted from

  • Ye, H.; Abdel-Ghany, S.E.; Anderson, T.D.; Pilon-Smits, E.A.; Pilon, M.
    CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation (2006), J. Biol. Chem., 281, 8958-8969.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information in presence of purified recombinant chloroplast protein CpSufE, Vmax value of cysteine desulfurase CpNifS increases over 40fold and the Km value towards cysteine decreases to half. CpSufE addition decreases the affinity of enzyme for selenocysteine, and a mutant CpSufE lacking the single cysteine is not able to stimulate CpNifS Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
L-cysteine 25°C, pH 7.4, presence of chloroplast protein CpSufE Arabidopsis thaliana
0.1
-
L-cysteine 25°C, pH 7.4 Arabidopsis thaliana
2.9
-
L-selenocysteine 25°C, pH 7.4 Arabidopsis thaliana
4.17
-
L-selenocysteine 25°C, pH 7.4, presence of chloroplast protein CpSufE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + CpNifS
-
Arabidopsis thaliana L-alanine + CpNifS-SSH
-
?
L-selenocysteine
-
Arabidopsis thaliana L-alanine + selenium
-
?

Subunits

Subunits Comment Organism
More enzyme CpNifS forms dynamic complexes with chloroplast protein CpSufE Arabidopsis thaliana