Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in presence of purified recombinant chloroplast protein CpSufE, Vmax value of cysteine desulfurase CpNifS increases over 40fold and the Km value towards cysteine decreases to half. CpSufE addition decreases the affinity of enzyme for selenocysteine, and a mutant CpSufE lacking the single cysteine is not able to stimulate CpNifS | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
L-cysteine | 25°C, pH 7.4, presence of chloroplast protein CpSufE | Arabidopsis thaliana | |
0.1 | - |
L-cysteine | 25°C, pH 7.4 | Arabidopsis thaliana | |
2.9 | - |
L-selenocysteine | 25°C, pH 7.4 | Arabidopsis thaliana | |
4.17 | - |
L-selenocysteine | 25°C, pH 7.4, presence of chloroplast protein CpSufE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + CpNifS | - |
Arabidopsis thaliana | L-alanine + CpNifS-SSH | - |
? | |
L-selenocysteine | - |
Arabidopsis thaliana | L-alanine + selenium | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme CpNifS forms dynamic complexes with chloroplast protein CpSufE | Arabidopsis thaliana |