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Literature summary for 2.8.1.7 extracted from

  • Zeng, J.; Zhang, Y.; Liu, Y.; Zhang, X.; Xia, L.; Liu, J.; Qiu, G.
    Expression, purification and characterization of a cysteine desulfurase, IscS, from Acidithiobacillus ferrooxidans (2007), Biotechnol. Lett., 29, 1983-1990.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Acidithiobacillus ferrooxidans
expression in Escherichia coli BL21 (DE3) Acidithiobacillus ferrooxidans

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of structure Acidithiobacillus ferrooxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe-S cluster Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
recombinant IscS by SDS-PAGE Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Acidithiobacillus ferrooxidans enzyme is involved in the iron-sulfur cluster assembly ?
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-
Acidithiobacillus ferrooxidans
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strain ATCC 23270, expression in Escherichia coli BL21 (DE3)
-

Purification (Commentary)

Purification (Comment) Organism
by one-step affinity chromatography to homogeneity Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.082
-
pH 8.0, 50°C, rate of production of thiocyanate Acidithiobacillus ferrooxidans
82500
-
pH 8.0, 50°C Acidithiobacillus ferrooxidans

Storage Stability

Storage Stability Organism
4°C, purified protein can be stored one month without loss of activity Acidithiobacillus ferrooxidans
at 4°C, 1 month, without significant change of activity Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine
-
Acidithiobacillus ferrooxidans L-alanine + H2S or formation of elemental sulfur, depending of presence of a reducing agent in the reaction mixture ?
L-cysteine + ? catalyzes the elimination of S from L-cysteine to yield L-alanine and elemental sulfur or H2S, depending on whether or not a reducing agent is added to the reaction mixture, provides sulfur for the assembly of iron–sulfur cluster Acidithiobacillus ferrooxidans L-alanine + ?
-
?
additional information enzyme is involved in the iron-sulfur cluster assembly Acidithiobacillus ferrooxidans ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE and modeling of structure Acidithiobacillus ferrooxidans
monomer 46000 Acidithiobacillus ferrooxidans

Synonyms

Synonyms Comment Organism
cysteine desulfurase
-
Acidithiobacillus ferrooxidans
IscS
-
Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Acidithiobacillus ferrooxidans