Cloned (Comment) | Organism |
---|---|
- |
Acidithiobacillus ferrooxidans |
expression in Escherichia coli BL21 (DE3) | Acidithiobacillus ferrooxidans |
Crystallization (Comment) | Organism |
---|---|
modeling of structure | Acidithiobacillus ferrooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe-S cluster | Acidithiobacillus ferrooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
recombinant IscS by SDS-PAGE | Acidithiobacillus ferrooxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acidithiobacillus ferrooxidans | enzyme is involved in the iron-sulfur cluster assembly | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Acidithiobacillus ferrooxidans | - |
strain ATCC 23270, expression in Escherichia coli BL21 (DE3) | - |
Purification (Comment) | Organism |
---|---|
by one-step affinity chromatography to homogeneity | Acidithiobacillus ferrooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.082 | - |
pH 8.0, 50°C, rate of production of thiocyanate | Acidithiobacillus ferrooxidans |
82500 | - |
pH 8.0, 50°C | Acidithiobacillus ferrooxidans |
Storage Stability | Organism |
---|---|
4°C, purified protein can be stored one month without loss of activity | Acidithiobacillus ferrooxidans |
at 4°C, 1 month, without significant change of activity | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine | - |
Acidithiobacillus ferrooxidans | L-alanine + H2S | or formation of elemental sulfur, depending of presence of a reducing agent in the reaction mixture | ? | |
L-cysteine + ? | catalyzes the elimination of S from L-cysteine to yield L-alanine and elemental sulfur or H2S, depending on whether or not a reducing agent is added to the reaction mixture, provides sulfur for the assembly of ironsulfur cluster | Acidithiobacillus ferrooxidans | L-alanine + ? | - |
? | |
additional information | enzyme is involved in the iron-sulfur cluster assembly | Acidithiobacillus ferrooxidans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE and modeling of structure | Acidithiobacillus ferrooxidans |
monomer | 46000 | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
cysteine desulfurase | - |
Acidithiobacillus ferrooxidans |
IscS | - |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Acidithiobacillus ferrooxidans |