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Literature summary for 2.8.1.7 extracted from

  • Muhlenhoff, U.; Balk, J.; Richhardt, N.; Kaiser, J.T.; Sipos, K.; Kispal, G.; Lill, R.
    Functional characterization of the eukaryotic cysteine desulfurase Nfs1p from Saccharomyces cerevisiae (2004), J. Biol. Chem., 279, 36906-36915.
    View publication on PubMed
Search for more literature for 2.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressiion in Escherichia coli Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol low activity Saccharomyces cerevisiae 5829
-
mitochondrion mitochondrial Nfs1p is required for the biogenesis of both mitochondrial and extramitochondrial Fe/S proteins Saccharomyces cerevisiae 5739
-
nucleus low activity Saccharomyces cerevisiae 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47300
-
 deduced molcular mass Saccharomyces cerevisiae
102000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + enzyme-cysteine Saccharomyces cerevisiae involved in the formation of Fe-S proteins L-alanine + enzyme-S-sulfanylcysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 W303-1A, Gal-NFS1, Gal-ATM1, Gal-YAH1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0127
-
 cysteine to DTT Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + enzyme-cysteine
-
 Saccharomyces cerevisiae L-alanine + enzyme-S-sulfanylcysteine
-
 ?
L-cysteine + enzyme-cysteine involved in the formation of Fe-S proteins Saccharomyces cerevisiae L-alanine + enzyme-S-sulfanylcysteine
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, gel filtration Saccharomyces cerevisiae
More N-terminal beta-strand of residues 99-104 is essential for the function of Nfs1p Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Nfs1p
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Saccharomyces cerevisiae