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Literature summary for 2.8.1.7 extracted from

  • Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N.
    Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions (2000), J. Biochem., 127, 559-567.
    View publication on PubMed
Search for more literature for 2.8.1.7

Activating Compound

Activating Compound Comment Organism Structure
pyruvate increases activity of CSD towards L-selenocysteine, but not towards L-cysteine Escherichia coli

Protein Variants

Protein Variants Comment Organism
C328A IscS mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected Escherichia coli
C358A CSD mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected Escherichia coli
C364A CsdB mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 L-selenocysteine pH 8, IcsS in the presence of pyruvate Escherichia coli
1.4
-
 L-selenocysteine pH 8, CSD in the presence of pyruvate Escherichia coli
2.6
-
 L-selenocysteine pH 8, CsdB in the presence of pyruvate Escherichia coli
3.5
-
 L-cysteine sulfinic acid pH 8, CSD in the presence of pyruvate Escherichia coli
26
-
 L-cysteine sulfinic acid pH 8, IcsS in the absence of pyruvate Escherichia coli
30
-
 L-cysteine sulfinic acid pH 8, CsdB in the presence of pyruvate Escherichia coli
39
-
 L-cysteine sulfinic acid pH 8, CsdB in the absence of pyruvate Escherichia coli
190
-
 L-cysteine sulfinic acid pH 8, IcsS in the presence of pyruvate Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + [enzyme]-cysteine Escherichia coli
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
three homologs: CSD, CsdB and IscS Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + [enzyme]-cysteine
-
 Escherichia coli L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine sulfinic acid
-
 Escherichia coli L-alanine + sulfite
-
 ?
L-selenocysteine
-
 Escherichia coli L-alanine + selenium
-
 ?