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Literature summary for 2.8.1.7 extracted from

  • Kato, S.; Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N.
    Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation (2000), Biosci. Biotechnol. Biochem., 64, 2412-2419.
    View publication on PubMed
Search for more literature for 2.8.1.7

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol increase of enzyme activity at 50 mM Synechocystis sp.
pyruvate increase of enzyme activity at 5 mM Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
0.43
-
 L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.
0.59
-
 L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
17
-
 L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron [Fe2-S2]ferredoxin Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Synechocystis sp.
87000
-
 isoenzyme SsCsd1, gel filtration Synechocystis sp.
88000
-
 isoenzyme SsCsd2, gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + [enzyme]-cysteine Synechocystis sp.
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 PCC6803
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme SsCsd1, isoenzyme SsCsd2 and isoenzyme SsCsd3 Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 isoenzyme SsCsd1, with L-cysteine sulfinic acid as substrate Synechocystis sp.
4.8
-
 isoenzyme SsCsd2, with L-cysteine sulfinic acid as substrate Synechocystis sp.
5.8
-
 isoenzyme SsCsd2, with L-selenocysteine as substrate Synechocystis sp.
21
-
 isoenzyme SsCsd1, with L-selenocysteine as substrate Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + [enzyme]-cysteine
-
 Synechocystis sp. L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine + [enzyme]-cysteine L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not Synechocystis sp. L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
L-cysteine sulfinic acid L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not Synechocystis sp. L-alanine + sulfite
-
 ?
L-cystine
-
 Synechocystis sp. ?
-
 ?
L-selenocysteine L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not Synechocystis sp. L-alanine + selenium
-
 ?
L-selenocystine
-
 Synechocystis sp. ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
 L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.
3.4
-
 L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
4
-
 L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
15
-
 L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 isoenzyme SsCsd1 with L-selenocysteine and isoenzyme SsCsd2 with L-selenocysteine and L-cysteine sulfinic acid as substrates Synechocystis sp.
7.7
-
 isoenzyme SsCsd1 with S-cysteine sulfinic acid as substrate Synechocystis sp.