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Literature summary for 2.8.1.6 extracted from
- Purification and characterization of biotin synthases (1997), Methods Enzymol., 279, 349-356.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Lysinibacillus sphaericus |  |
| S-adenosyl-L-methionine | required | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | presence of a [2Fe-2S] cluster | Escherichia coli | |
| Iron | presence of a [2Fe-2S] cluster | Lysinibacillus sphaericus | |
| Iron | the 104000 Da dimeric enzyme form contains a single [2Fe-2S] cluster per dimer | Escherichia coli | |
| Iron | the 82000 Da dimeric enzyme form contains one [2Fe-2S]cluster per monomer | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE | Escherichia coli |
| 82000 | - |
at least 3 forms of the enzyme: 82000 Da, 104000 Da, 160000 Da | Escherichia coli |
| 104000 | - |
at least 3 forms of the enzyme: 82000 Da, 104000 Da, 160000 Da | Escherichia coli |
| 160000 | - |
at least 3 forms of the enzyme: 82000 Da, 104000 Da, 160000 Da | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dethiobiotin + sulfur | Escherichia coli | catalyzes the last step of the biosynthesis of biotin | biotin | - |
? | |
| dethiobiotin + sulfur | Lysinibacillus sphaericus | catalyzes the last step of the biosynthesis of biotin | biotin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Lysinibacillus sphaericus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin | radical chemistry is involved in the mechanism of biotin synthase | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dethiobiotin + sulfur | - |
Escherichia coli | biotin | - |
? | |
| dethiobiotin + sulfur | - |
Lysinibacillus sphaericus | biotin | - |
? | |
| dethiobiotin + sulfur | catalyzes the last step of the biosynthesis of biotin | Escherichia coli | biotin | - |
? | |
| dethiobiotin + sulfur | catalyzes the last step of the biosynthesis of biotin | Lysinibacillus sphaericus | biotin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Lysinibacillus sphaericus |
| dimer | 2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE | Escherichia coli |
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