Any feedback?
Literature summary for 2.8.1.5 extracted from
- Thiosulfate reductase from a moderately thermophilic iron-oxidizing bacterium, strain TI-1: purification and characterization (1997), Biosci. Biotechnol. Biochem., 61, 470-474.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
thermophilic iron-oxidizing bacterium strain TI-1 |  |
| o-phenanthroline | - |
thermophilic iron-oxidizing bacterium strain TI-1 | |
| Sodium cyanide | 2.5fold increase at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzene sufonic acid | strong inhibition at 0.1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
| Co2+ | slight inhibition at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
| Ni2+ | slight inhibition at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
| Zn2+ | slight inhibition at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.125 | - |
NADH | pH 6, 45°C | thermophilic iron-oxidizing bacterium strain TI-1 | |
| 0.29 | - |
thiosulfate | pH 6, 45°C | thermophilic iron-oxidizing bacterium strain TI-1 | |
| 5 | - |
NADPH | pH 6, 45°C | thermophilic iron-oxidizing bacterium strain TI-1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
thermophilic iron-oxidizing bacterium strain TI-1 | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
homotetramer, 4 * 58000, SDS-PAGE | thermophilic iron-oxidizing bacterium strain TI-1 |
| 243000 | - |
gel filtration | thermophilic iron-oxidizing bacterium strain TI-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| thermophilic iron-oxidizing bacterium strain TI-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
thermophilic iron-oxidizing bacterium strain TI-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + NAD(P)H | - |
thermophilic iron-oxidizing bacterium strain TI-1 | H2S + NAD(P)+ + sulfite | - |
? | |
| thiosulfate + NADH | - |
thermophilic iron-oxidizing bacterium strain TI-1 | H2S + NAD+ + sulfite | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | homotetramer, 4 * 58000, SDS-PAGE | thermophilic iron-oxidizing bacterium strain TI-1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
thermophilic iron-oxidizing bacterium strain TI-1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
no enzyme activity after 15 min | thermophilic iron-oxidizing bacterium strain TI-1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
thermophilic iron-oxidizing bacterium strain TI-1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
