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Literature summary for 2.8.1.5 extracted from
- Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca (1984), Planta, 162, 243-249.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thiosulfate | above 0.5 mM | [Chlorella] fusca |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.156 | - |
thiosulfate | pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II | [Chlorella] fusca | |
| 0.164 | - |
thiosulfate | pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I | [Chlorella] fusca | |
| 1.54 | - |
dithioerythritol | pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II | [Chlorella] fusca | |
| 3.1 | - |
dithioerythritol | pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I | [Chlorella] fusca | |
| 20 | - |
KCN | pH 9, 37°C, with dithioerythritol as second substrate | [Chlorella] fusca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
isoenzyme IIIb, gel filtration | [Chlorella] fusca |
| 26500 | - |
isoenzyme II, gel filtration | [Chlorella] fusca |
| 28000 | - |
isoenzyme I, gel filtration | [Chlorella] fusca |
| 55000 | - |
isoenzyme IIIa, gel filtration | [Chlorella] fusca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + 2 dithioerythritol | [Chlorella] fusca | - |
sulfite + dithioerythritol disulfide + sulfide | - |
? | |
| thiosulfate + 2 dithioerythritol | [Chlorella] fusca 211-8b | - |
sulfite + dithioerythritol disulfide + sulfide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Chlorella] fusca | - |
- |
- |
| [Chlorella] fusca 211-8b | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb | [Chlorella] fusca |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
[Chlorella] fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanate + dithioerythritol | activity only in presence of thiols | [Chlorella] fusca | ? | - |
? | |
| cyanate + dithioerythritol | activity only in presence of thiols | [Chlorella] fusca 211-8b | ? | - |
? | |
| KCN + dithioerythritol | - |
[Chlorella] fusca | ? | - |
? | |
| KCN + dithioerythritol | - |
[Chlorella] fusca 211-8b | ? | - |
? | |
| additional information | little activity with: glutathione, L-cysteine, beta-mercaptoethanol | [Chlorella] fusca | ? | - |
? | |
| additional information | little activity with: glutathione, L-cysteine, beta-mercaptoethanol | [Chlorella] fusca 211-8b | ? | - |
? | |
| thiosulfate + 2 dithioerythritol | - |
[Chlorella] fusca | sulfite + dithioerythritol disulfide + sulfide | - |
? | |
| thiosulfate + 2 dithioerythritol | - |
[Chlorella] fusca 211-8b | sulfite + dithioerythritol disulfide + sulfide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | [Chlorella] fusca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
isoenzyme TSR IIIa | [Chlorella] fusca |
| 9 | - |
isoenzyme TSRI and TSR II | [Chlorella] fusca |
| 9.5 | - |
isoenzyme TSR IIIb | [Chlorella] fusca |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | stimulates thiosulfate reductase IIIa and IIIb | [Chlorella] fusca | |
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