Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.1.2 extracted from

  • Yadav, P.K.; Yamada, K.; Chiku, T.; Koutmos, M.; Banerjee, R.
    Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase (2013), J. Biol. Chem., 288, 20002-20013.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 and 0.02 M betaine hydrochloride Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 thioredoxin in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.02
-
 3-Mercaptopyruvate glutathione as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.022
-
 3-Mercaptopyruvate using L-cysteine as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.025
-
 3-Mercaptopyruvate using dihydrolipioic acid as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.026
-
 3-Mercaptopyruvate using dithiothreitol as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.03
-
 3-Mercaptopyruvate using L-homocysteine as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.13
-
 3-Mercaptopyruvate using 2-mercaptoethanol as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.35
-
 3-Mercaptopyruvate using cyanide as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.35
-
 3-Mercaptopyruvate using thioredoxin as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
4.1
-
 L-cysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
4.4
-
 dihydrolipoic acid in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
4.6
-
 dithiothreitol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
6
-
 cyanide in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
12.5
-
 L-homocysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
28
-
 glutathione in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
108
-
 2-mercaptoethanol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-mercaptopyruvate + cyanide Homo sapiens
-
 pyruvate + thiocyanate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P25325
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopyruvate + 2-mercaptoethanol
-
 Homo sapiens ?
-
 ?
3-mercaptopyruvate + cyanide
-
 Homo sapiens pyruvate + thiocyanate
-
 ?
3-mercaptopyruvate + dihydrolipoic acid
-
 Homo sapiens ?
-
 ?
3-mercaptopyruvate + dithiothreitol
-
 Homo sapiens ?
-
 ?
3-mercaptopyruvate + glutathione
-
 Homo sapiens ?
-
 ?
3-mercaptopyruvate + L-cysteine
-
 Homo sapiens ?
-
 ?
3-mercaptopyruvate + L-homocysteine
-
 Homo sapiens ?
-
 ?
3-mercaptopyruvate + thioredoxin thioredoxin is the preferred persulfide acceptor Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
mercaptopyruvate sulfurtransferase
-
 Homo sapiens
MST
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 glutathione in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.8
-
 L-homocysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
1.1
-
 L-cysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
1.3
-
 thioredoxin in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
1.7
-
 dihydrolipoic acid in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.4
-
 cyanide in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
6.1
-
 dithiothreitol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
229
-
 2-mercaptoethanol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 glutathione in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.06
-
 L-homocysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.27
-
 L-cysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.39
-
 dihydrolipoic acid in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
0.4
-
 cyanide in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
1.3
-
 dithiothreitol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.1
-
 2-mercaptoethanol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
520
-
 thioredoxin in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens