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Literature summary for 2.8.1.2 extracted from
- Regulation of mercaptopyruvate sulfurtransferase activity via intrasubunit and intersubunit redox-sensing switches (2013), Antioxid. Redox Signal., 19, 1792-1802.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | rat-reduced thioredoxin activates the enzyme 2.3fold after treatment with dithiothreitol, but dithiothreitol does not increase enzyme activity. The Escherichia coli thioredoxin-thioredoxin reductase-NADPH system more effectively increases enzyme activity to 4.5fold that of the control than the rat thioredoxin-thioredoxin reductase-NADPH system, which increases enzyme activity to 3fold that of the control | Mus musculus |  |
| thioredoxin | rat-reduced thioredoxin activates the enzyme 2.3fold after treatment with dithiothreitol, but dithiothreitol does not increase enzyme activity. The Escherichia coli thioredoxin-thioredoxin reductase-NADPH system more effectively increases enzyme activity to 4.5fold that of the control than the rat thioredoxin-thioredoxin reductase-NADPH system, which increases enzyme activity to 3fold that of the control | Rattus norvegicus | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C154S | active site mutant | Mus musculus |
| C154S | active site mutant | Rattus norvegicus |
| C263S | active site mutant | Mus musculus |
| C263S | active site mutant | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | - |
Mus musculus | |
| iodoacetate | - |
Rattus norvegicus | |
| tetrathionate | reduced GSH and GSH together with the reducing system partially restore the activity of tetrathionate-inhibited enzyme. Enzyme that is oxidized by an excess molar dose of tetrathionate is completely reactivated by dithiothreitol, reduced thioredoxin, and thioredoxin together with the reducing system | Mus musculus | |
| tetrathionate | reduced GSH and GSH together with the reducing system partially restore the activity of tetrathionate-inhibited enzyme. Enzyme that is oxidized by an excess molar dose of tetrathionate is completely reactivated by dithiothreitol, reduced thioredoxin, and thioredoxin together with the reducing system | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32800 | - |
2 * 32800, calculated from amino acid sequence | Mus musculus |
| 32800 | - |
2 * 32800, calculated from amino acid sequence | Rattus norvegicus |
| 33000 | - |
2 * 33000, SDS-PAGE | Mus musculus |
| 33000 | - |
2 * 33000, SDS-PAGE | Rattus norvegicus |
| 66600 | - |
gel filtration | Mus musculus |
| 66600 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + cyanide | Mus musculus | - |
pyruvate + thiocyanate | - |
? | |
| 3-mercaptopyruvate + cyanide | Rattus norvegicus | - |
pyruvate + thiocyanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + cyanide | - |
Mus musculus | pyruvate + thiocyanate | - |
? | |
| 3-mercaptopyruvate + cyanide | - |
Rattus norvegicus | pyruvate + thiocyanate | - |
? | |
| additional information | in the catalytic process of the enzyme, hydrogen peroxide is possibly produced by persulfide of the sulfur-accepted substrate and sulfur oxides are possibly produced in the redox cycle of persulfide formed at the catalytic site cysteine of the reaction intermediate | Mus musculus | ? | - |
? | |
| additional information | in the catalytic process of the enzyme, hydrogen peroxide is possibly produced by persulfide of the sulfur-accepted substrate and sulfur oxides are possibly produced in the redox cycle of persulfide formed at the catalytic site cysteine of the reaction intermediate | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 33000, SDS-PAGE | Mus musculus |
| homodimer | 2 * 33000, SDS-PAGE | Rattus norvegicus |
| homodimer | 2 * 32800, calculated from amino acid sequence | Mus musculus |
| homodimer | 2 * 32800, calculated from amino acid sequence | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mercaptopyruvate sulfurtransferase | - |
Mus musculus |
| mercaptopyruvate sulfurtransferase | - |
Rattus norvegicus |
| MST | - |
Mus musculus |
| MST | - |
Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | mercaptolactate-cysteine disulfiduria is caused by enzyme defect with or without mental retardation | Mus musculus |
| physiological function | the enzyme serves not only as an enzyme in cysteine catabolism, but also as an antioxidant protein | Mus musculus |
| physiological function | the enzyme serves not only as an enzyme in cysteine catabolism, but also as an antioxidant protein | Rattus norvegicus |
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