Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,4-dithiothreitol | - |
Rattus norvegicus | |
dithiothreitol | activates MST chiefly via reduction of a sulfenyl Cys247 | Rattus norvegicus | |
additional information | reduced glutathione does not affect MST activity | Rattus norvegicus | |
thioredoxin | Escherichia coli and rat thioredoxin activate the enzyme to 2.3- and 4.9fold the levels of activation by dithiothreitol-treated and -untreated enzyme, resp. Activation occurs via cleavage of the intersubunit bonds of the enzyme dimer at C154 and C263. Escherichia coli thioredoxin with substitution C35S forms adducts with the enzyme and activates after treatment with dithiotreitol | Rattus norvegicus | |
thioredoxin | Escherichia coli or rat reduced thioredoxin (Trx) cleaves the intersubunit disulfide bond to activate MST to 2.3- and 4.9fold the levels of activation of dithiothreitol (DTT)-treated and DTT-untreated MST, respectively. An intersubunit disulfide bond serves as a redox switch for enzyme activation | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C154S | site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST | Rattus norvegicus |
C254S | site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, activation with reduced thioredoxin | Rattus norvegicus |
C263S | site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST | Rattus norvegicus |
C64S | site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, activation with reduced thioredoxin | Rattus norvegicus |
additional information | C154S/C263S mutant, site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32800 | - |
monomer, gel filtration HPLC analysis, MST exhibits monomer-dimer equilibrium, the monomer to dimer ratio is nearly 9 to 1 in 0.2 M potassium phosphate buffer, pH 7.0 | Rattus norvegicus |
66600 | - |
dimer, gel filtration HPLC analysis, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | MST contributes to maintain redox homeostasis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | P97532 | expression in Escherichia coli BL21 (DE3) | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MST contributes to maintain redox homeostasis | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2*32800, HPLC, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond | Rattus norvegicus |
monomer | 32800, HPLC, MST exhibits monomer-dimer equilibrium | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
3-mercaptopyruvate sulfurtransferase | - |
Rattus norvegicus |
mercaptopyruvate sulfurtransferase | - |
Rattus norvegicus |
MST | - |
Rattus norvegicus |