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Literature summary for 2.8.1.2 extracted from

  • Nagahara, N.; Yoshii, T.; Abe, Y.; Matsumura, T.
    Thioredoxin-dependent enzymatic activation of mercaptopyruvate sulfurtransferase. An intersubunit disulfide bond serves as a redox switch for activation (2007), J. Biol. Chem., 282, 1561-1569.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,4-dithiothreitol
-
Rattus norvegicus
dithiothreitol activates MST chiefly via reduction of a sulfenyl Cys247 Rattus norvegicus
additional information reduced glutathione does not affect MST activity Rattus norvegicus
thioredoxin Escherichia coli and rat thioredoxin activate the enzyme to 2.3- and 4.9fold the levels of activation by dithiothreitol-treated and -untreated enzyme, resp. Activation occurs via cleavage of the intersubunit bonds of the enzyme dimer at C154 and C263. Escherichia coli thioredoxin with substitution C35S forms adducts with the enzyme and activates after treatment with dithiotreitol Rattus norvegicus
thioredoxin Escherichia coli or rat reduced thioredoxin (Trx) cleaves the intersubunit disulfide bond to activate MST to 2.3- and 4.9fold the levels of activation of dithiothreitol (DTT)-treated and DTT-untreated MST, respectively. An intersubunit disulfide bond serves as a redox switch for enzyme activation Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C154S site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST Rattus norvegicus
C254S site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, activation with reduced thioredoxin Rattus norvegicus
C263S site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST Rattus norvegicus
C64S site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, activation with reduced thioredoxin Rattus norvegicus
additional information C154S/C263S mutant, site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32800
-
monomer, gel filtration HPLC analysis, MST exhibits monomer-dimer equilibrium, the monomer to dimer ratio is nearly 9 to 1 in 0.2 M potassium phosphate buffer, pH 7.0 Rattus norvegicus
66600
-
dimer, gel filtration HPLC analysis, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus MST contributes to maintain redox homeostasis ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus P97532 expression in Escherichia coli BL21 (DE3)
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MST contributes to maintain redox homeostasis Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer 2*32800, HPLC, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond Rattus norvegicus
monomer 32800, HPLC, MST exhibits monomer-dimer equilibrium Rattus norvegicus

Synonyms

Synonyms Comment Organism
3-mercaptopyruvate sulfurtransferase
-
Rattus norvegicus
mercaptopyruvate sulfurtransferase
-
Rattus norvegicus
MST
-
Rattus norvegicus