Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C247S | no enzyme activity | Rattus norvegicus |
C248R | slightly increased Km | Rattus norvegicus |
R178G | increased Km | Rattus norvegicus |
R196G | increased Km | Rattus norvegicus |
R248G | facilitated catalysis of thiosulfate | Rattus norvegicus |
S249A | no significant difference in Km compared to wild-type enzyme | Rattus norvegicus |
S249K | facilitated catalysis of thiosulfate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
3-Mercaptopyruvate | S249A mutant | Rattus norvegicus | |
1.1 | - |
3-Mercaptopyruvate | S249K mutant | Rattus norvegicus | |
1.2 | - |
3-Mercaptopyruvate | wild-type enzyme | Rattus norvegicus | |
2.1 | - |
3-Mercaptopyruvate | G248R mutant | Rattus norvegicus | |
11 | - |
3-Mercaptopyruvate | R196G mutant | Rattus norvegicus | |
70 | - |
3-Mercaptopyruvate | R187G mutant | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | Rattus norvegicus | - |
pyruvate + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P97532 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | - |
Rattus norvegicus | pyruvate + thiocyanate | - |
? |