Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.1.2 extracted from

  • Magahara, N.; Nishino, T.
    Role of amino acid residues in the active site of rat liver mercaptopyruvate sulfurtransferase. CDNA cloning, overexpression, and site-directed mutagenesis (1996), J. Biol. Chem., 271, 27395-27401.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C247S no enzyme activity Rattus norvegicus
C248R slightly increased Km Rattus norvegicus
R178G increased Km Rattus norvegicus
R196G increased Km Rattus norvegicus
R248G facilitated catalysis of thiosulfate Rattus norvegicus
S249A no significant difference in Km compared to wild-type enzyme Rattus norvegicus
S249K facilitated catalysis of thiosulfate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
3-Mercaptopyruvate S249A mutant Rattus norvegicus
1.1
-
3-Mercaptopyruvate S249K mutant Rattus norvegicus
1.2
-
3-Mercaptopyruvate wild-type enzyme Rattus norvegicus
2.1
-
3-Mercaptopyruvate G248R mutant Rattus norvegicus
11
-
3-Mercaptopyruvate R196G mutant Rattus norvegicus
70
-
3-Mercaptopyruvate R187G mutant Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-mercaptopyruvate + cyanide Rattus norvegicus
-
pyruvate + thiocyanate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P97532
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopyruvate + cyanide
-
Rattus norvegicus pyruvate + thiocyanate
-
?