Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Thermus thermophilus |
expressed in Escherichia coli Rosetta DE3 | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method at 208C, using 1 microl of sample solution mixed with an equal volume of reservoir solution, composed of 100 mM BicineNaOH buffer (pH 9.0) containing 200 mM NaCl, 12% (w/v) polyethylene glycol(PEG) 3350, and 010% (v/v) N,N-dimethylformamide. The native crystal belongs to the space group P43212, with unit cell parameters of a 5 b 5 70.1 Angstrom and c 5 128.6 Angstrom | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
C130S | ca. 10% of wild-type activity | Thermus thermophilus |
C133S | ca. 1% of wild-type activity | Thermus thermophilus |
C222S | ca. 5-7% of wild-type activity | Thermus thermophilus |
D161A | no activity detected | Thermus thermophilus |
D59A | no activity detected | Thermus thermophilus |
E203A | ca. 20% of wild-type activity | Thermus thermophilus |
H157A | ca. 80% of wild-type activity | Thermus thermophilus |
K58A | ca. 62% of wild-type activity | Thermus thermophilus |
N158A | ca. 75% of wild-type activity | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | each subunit contains tow N- and c-terminal zinc fingers | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35600 | - |
- |
Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Pyrococcus horikoshii | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Pyrococcus horikoshii DSM 12428 | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus DSM 7039 | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58038 | - |
- |
Pyrococcus horikoshii DSM 12428 | O58038 | - |
- |
Thermus thermophilus | Q72LF3 | - |
- |
Thermus thermophilus DSM 7039 | Q72LF3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Storage Stability | Organism |
---|---|
-80°C, pH 7.0, protein concentration 10 mg/ml | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Pyrococcus horikoshii | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Pyrococcus horikoshii DSM 12428 | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus DSM 7039 | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35366, calculated from aminoa acid sequence | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
PH0300 | - |
Pyrococcus horikoshii |
TtuA | - |
Thermus thermophilus |
TtuA | - |
Pyrococcus horikoshii |
TT_C0106 | - |
Thermus thermophilus |
Veg136 protein | - |
Thermus thermophilus |