Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus DSM 7039 | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72LF3 | - |
- |
Thermus thermophilus DSM 7039 | Q72LF3 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q72LF3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus DSM 7039 | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TtuA | - |
Thermus thermophilus |
TT_C0106 | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | thiolation increases 7fold when the temperature rises from 50°C to 70°C | Thermus thermophilus |
Organism | Comment | Expression |
---|---|---|
Thermus thermophilus | enzyme expression increases with increasing the temperature from 50°C to 80°C | up |
General Information | Comment | Organism |
---|---|---|
metabolism | 2-Thioribothymidine (s2T) is a modified nucleoside of U, specifically found at position 54 of tRNAs from extreme thermophilic microorganisms. The function of the 2-thiocarbonyl group of s2T54 is thermostabilization of the three-dimensional structure of tRNA | Thermus thermophilus |
physiological function | the sulfur atom of 2-thioribothymidine in tRNA is derived from cysteine or sulfate. Specific 2-thiolation of ribothymidine, at position 54, is observed in vitro, in the presence of ATP. The 2-thiolation reaction in vitro as well as expression of 2-thiolation enzymes in vivo show strong temperature dependence | Thermus thermophilus |