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Literature summary for 2.8.1.1 extracted from

  • Pagani, S.; Forlani, F.; Carpen, A.; Bordo, D.; Colnaghi, R.
    Mutagenic analysis of Thr-232 in rhodanese from Azotobacter vinelandii highlighted the differences of this prokaryotic enzyme from the known sulfurtransferases (2000), FEBS Lett., 472, 307-311.
    View publication on PubMed
Search for more literature for 2.8.1.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli M15 Azotobacter vinelandii

Protein Variants

Protein Variants Comment Organism
K249A complete inactivation of rhodanese with thiosulfate Bos taurus
T232A increase in thiosulfate-cyanide sulfurtransferase activity and no activity with 3-mercaptopyruvate as sulfur donor Azotobacter vinelandii
T232K increase in thiosulfate-cyanide sulfurtransferase activity and no activity with 3-mercaptopyruvate as sulfur donor Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?
thiosulfate + cyanide Azotobacter vinelandii
-
 sulfite + thiocyanate
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter vinelandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
225
-
 recombinant wild-type enzyme Azotobacter vinelandii
440
-
 T232A Azotobacter vinelandii
550
-
 T232K Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?
thiosulfate + cyanide
-
 Azotobacter vinelandii sulfite + thiocyanate
-
 ?