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Literature summary for 2.8.1.1 extracted from

  • Miller, D.M.; Kurzban, G.P.; Mendoza, J.A.; Chirgwin, J.M.; Hardies, S.C.; Horowitz, P.M.
    Recombinant bovine rhodanese: purification and comparison with bovine liver rhodanese (1992), Biochim. Biophys. Acta, 1121, 286-292.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.9
-
thiosulfate liver rhodanese Bos taurus
16.8
-
thiosulfate recombinant rhodanese Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, bovine liver and recombinant Escherichia coli BL21(DE3) enzyme, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Bos taurus
-
sulfite + thiocyanate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
bovine liver and recombinant Escherichia coli BL21(DE3) enzyme
-

Purification (Commentary)

Purification (Comment) Organism
bovine liver and recombinant Escherichia coli BL21(DE3) enzyme Bos taurus

Renatured (Commentary)

Renatured (Comment) Organism
refolds from 8 M urea to enzymatically active species Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
730
-
purificated liver rhodanese Bos taurus
816
-
washed precipitate of recombinant rhodanese Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + cyanide
-
Bos taurus sulfite + thiocyanate
-
?

Subunits

Subunits Comment Organism
? x * 33000, bovine liver and recombinant Escherichia coli BL21(DE3) enzyme, SDS-PAGE Bos taurus