Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.9 | - |
thiosulfate | liver rhodanese | Bos taurus | |
16.8 | - |
thiosulfate | recombinant rhodanese | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, bovine liver and recombinant Escherichia coli BL21(DE3) enzyme, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + cyanide | Bos taurus | - |
sulfite + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine liver and recombinant Escherichia coli BL21(DE3) enzyme | - |
Purification (Comment) | Organism |
---|---|
bovine liver and recombinant Escherichia coli BL21(DE3) enzyme | Bos taurus |
Renatured (Comment) | Organism |
---|---|
refolds from 8 M urea to enzymatically active species | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
730 | - |
purificated liver rhodanese | Bos taurus |
816 | - |
washed precipitate of recombinant rhodanese | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + cyanide | - |
Bos taurus | sulfite + thiocyanate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, bovine liver and recombinant Escherichia coli BL21(DE3) enzyme, SDS-PAGE | Bos taurus |