Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Listeria monocytogenes |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 8% (w/v) PEG3350 and 100 mM Bicine (pH 9.2) | Listeria monocytogenes |
Protein Variants | Comment | Organism |
---|---|---|
G527A | the mutant has much decreased rifampin-phosphorylation activity in vitro and reduced rifampin resistance in vivo | Listeria monocytogenes |
G527S | the mutant has much decreased rifampin-phosphorylation activity in vitro and reduced rifampin resistance in vivo | Listeria monocytogenes |
G527Y | the mutant has much decreased rifampin-phosphorylation activity in vitro and reduced rifampin resistance in vivo | Listeria monocytogenes |
H825A | the mutant loses the ability to phosphorylate rifampin both in vitro and in vivo | Listeria monocytogenes |
K670A | the mutant loses the ability to phosphorylate rifampin both in vitro and in vivo | Listeria monocytogenes |
Q337A | the mutant has significantly reduced activity compared to the wild type enzyme | Listeria monocytogenes |
R666A | the mutant loses the ability to phosphorylate rifampin both in vitro and in vivo | Listeria monocytogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Listeria monocytogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rifampicin + H2O | Listeria monocytogenes | - |
AMP + 21-phosphorifampicin + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Listeria monocytogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rifampicin + H2O | - |
Listeria monocytogenes | AMP + 21-phosphorifampicin + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rifampin phosphotransferase | - |
Listeria monocytogenes |
RPH | - |
Listeria monocytogenes |