Cloned (Comment) | Organism |
---|---|
the starch-binding domain, SBD, Asp68-Gly184, of glucan, water dikinase 3 is cloned into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells, the region encoding residues 1-201, containing the chloroplast transit peptide and the SBD is fused to YFP and transiently expressed in Nicotiana benthamiana | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14200 | - |
theoretical, starch-binding domain, SBD, Asp68-Gly184, verified by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | GWD3-SBD shows strongest binding towards alpha-cyclodextrin and no detectable affinity for linear maltooligosaccharides or for phosphorylated beta-cyclodextrin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GWD3-SBD is purified on a beta-cyclodextrin Sepharose column and by an additional His-trap purification step | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | GWD3-SBD shows strongest binding towards alpha-cyclodextrin and no detectable affinity for linear maltooligosaccharides or for phosphorylated beta-cyclodextrin | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucan, water dikinase | - |
Arabidopsis thaliana |
glucan, water dikinase 3 | - |
Arabidopsis thaliana |
GWD3 | - |
Arabidopsis thaliana |